| Identification | Back Directory | [Name]
PFM01 | [CAS]
1558598-41-6 | [Synonyms]
PFM01
PFM01 >=98% (HPLC)
(5Z)-5-[(4-Hydroxyphenyl)methylene]-3-(2-methylpropyl)-2-thioxo-4-thiazolidinone | [Molecular Formula]
C14H15NO2S2 | [MDL Number]
MFCD02220972 | [MOL File]
1558598-41-6.mol | [Molecular Weight]
293.4 |
| Hazard Information | Back Directory | [Biochem/physiol Actions]
PFM01 is a cell-permeable N-alkylated Mirin (Sigma Cat. No. 475954) derivative that selectively inhibits against MRE11 endo-, but not exo-, nuclease activity. PFM01 targets MRE11 at a site near the dimer interface, distinct from that occupied by Mirin and PFM39 to allow disruption of the ssDNA-binding groove and selective inhibition against MRE11 endo-, but not exo-, nuclease activity. While both endonuclease and exonuclease activities are required for MRE11-mediated homologous recombination (HR) repair, only FM01 (100 μM), but not the exonuclease inhibitors Mirin (500 μM) and PFM39 (100 μM), rescues G2-phase double-strand break (DSB) repair defect in HR protein BRCA2-deficient HSC62-hTERT fibroblasts following ionizing irradiation (IR) by blocking HR initiation and thereby allowing non-homologous end joining (NHEJ) to proceed. | [storage]
Store at -20°C |
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Sigma-Aldrich
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BOC Sciences
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DC Chemicals
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Energy Chemical
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