| Identification | Back Directory | [Name]
H-LEU-4M-BETANA HCL | [CAS]
4467-68-9 | [Synonyms]
H-Leu-4MbNA·HCl
H-LEU-4M-BETANA HCL
LEUCINE-4M-BETANA HCL
L-LEU-4-METHOXY-BETA-NAPHTHYLAMIDE HCL
L-LEUCYL-4-METHOXY-B-NAPHTHYLAMIDE HYDROCHLORIDE
L-leucine 4-methoxy-B-naphthylamide*hydrochloride
L-Leucine 4-methoxy-β-naphthylamide hydrochloride
L-LEUCINE 4-METHOXY-BETA-NAPHTHYLAMIDE HYDROCHLORIDE
(S)-2-amino-N-(4-methoxy-2-naphthyl)-4-methylvaleramide monohydrochloride | [EINECS(EC#)]
224-735-3 | [Molecular Formula]
C17H23ClN2O2 | [MDL Number]
MFCD00034967 | [MOL File]
4467-68-9.mol | [Molecular Weight]
322.83 |
| Chemical Properties | Back Directory | [Appearance]
Crystalline | [storage temp. ]
2-8°C
| [solubility ]
≤25mg/ml in ethanol;10mg/ml in DMSO;20mg/ml in dimethyl formamide | [form ]
crystalline solid |
| Hazard Information | Back Directory | [Chemical Properties]
Crystalline | [Description]
L-Leucine 4-methoxy-β-naphthylamide is a cell-permeable substrate for aminopeptidase M and leucine aminopeptidase that was developed for intracellular analysis of protease activities. | [Uses]
L-Leucine 4-methoxy-β-naphthylamide is a cell-permeable substrate for aminopeptidase M and leucine aminopeptidase that was developed for intracellular analysis of protease activities. | [in vitro]
l-leucine 4-methoxy-β-naphthylamide was identified as a cell-permeable substrate for aminopeptidase m and leucine aminopeptidase that was developed for intracellular analysis of protease activities [1]. in a previous method-developing study, peptide derivatives of 4-methoxy-β-naphthylamine including l-leucine 4-methoxy-β-naphthylamide were incubated in microtiter plates together with nitrosalicylaldehyde. selectivity was achieved by running parallel assays containing inhibitors partially selective for each of peptide derivatives. this method was successfully validated by measurements in cells isolated from cathepsin b-/--, k-/--, and l-/-- mice [2]. | [References]
[1] monis, b. ,wasserkrug, h. and seligman, a.m. comparison of fixatives and substrates for aminopeptidase. journal of histochemistry and cytochemistry 13(6), 503-509 (1965).
[2] rüttger, a. ,mollenhauer, j.,lser, r., et al. microplate assay for quantitative determination of cathepsin activities in viable cells using derivatives of 4-methoxy-β-naphthylamide. biotechniques 41(4), 469-473 (2006). |
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| Company Name: |
Energy Chemical
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| Tel: |
021-58432009 400-005-6266 |
| Website: |
http://www.energy-chemical.com |
| Company Name: |
SIGMA-RBI
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800 736 3690 (Orders) |
| Website: |
www.sigma-aldrich.com |
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