188404-10-6
188404-10-6 结构式
基本信息
中文别名
化合物 T15948甲基花生四烯酸氟磷酸酯
((5Z,8Z,11Z,14Z)-二十碳-5,8,11,14-四烯-1-基)氟磷酸甲酯
英文别名
MAPFMAFP
Phosphonofluoridicacid
METHYL ARACHIDONYL FLUOROPHOSPHONATE
(5Z,8Z,11Z,14Z)-5,8,11,14-Eicosatetraenylmethylester
METHYLPHOSPHONOFLUORIDIC ACID 5,8,11,14-EICOSATETRAENYL ESTER
5Z,8Z,11Z,14Z-EICOSATETRAENYL-PHOSPHONOFLUORIDIC ACID, METHYL ESTER
Phosphonofluoridic acid, methyl-5Z,8Z,11Z,14Z-eicosatetraenyl ester
(all-Z)-5,8,11,14-Eicosatetraenylphosphonofluoridic Acid Methyl Ester
(5Z,8Z,11Z,14Z)-1-[fluoro(methoxy)phosphoryl]icosa-5,8,11,14-tetraene
物理化学性质
RTECS号TA8108000
闪点-16 °C
储存条件-70°C
溶解度DMF: 3 mg/ml; DMSO: 3 mg/ml; Ethanol: 3.5 mg/ml; Ethanol:PBS (pH 7.2) 1:1: .5 mg/ml; PBS (7.2): <1 mg/ml (colloidal suspension
形态methyl acetate solution
敏感性空气敏感
安全数据
常见问题列表
生物活性
MAFP (Methyl Arachidonyl Fluorophosphonate) 是选择性，针对活性位点，不可逆的 cPLA2 和 iPLA2 抑制剂。 MAFP也是一种有效的不可逆的 anandamide amidase 抑制剂。靶点
cPLA2, iPLA2, Anandamide amidase
体外研究
MAFP inhibits iPLA2, in a concentration-dependent manner with an IC 50 of 5 μM after a 5 min preincubation at 40°C in P388D1 cells. cPLA, is a phospholipid hydrolase using the hydroxyl of serine-228 residue as its catalytic nucleophile. MAFP is also an inhibitor of anandamide amidase and as a ligand for the CB1 cannabinoid receptor. MAFP demonstrates selectivity towards anandamide amidase for which it is approximately 3000 and 30000-fold more potent than it is towards chymotrypsin and trypsin, respectively. MAFP displaces [ 3 H]CP-55940 binding to the CB1 cannabinoid receptor with an IC 50 of 20 nM vs 40 nM for anandamide.