2503-26-6

中文名称绿僵菌素B, 来源于绿僵菌属

英文名称 destruxin B

CAS 2503-26-6

分子式 C30H51N5O7

分子量 593.76

MOL 文件 2503-26-6.mol

2503-26-6 结构式

基本信息物理化学性质安全数据绿僵菌素B, 来源于绿僵菌属价格(试剂级) 常见问题列表

基本信息

中文别名

绿僵菌素B
黑僵菌素 B
绿僵菌素B, 来源于绿僵菌属

英文别名

destruxin B
Destruxin B from Metarhizium anisopliae
Destruxin B, from Metarrhizium anisopliae
Destruxin B, 97.5%, from Metarrhizium anisopliae
N-[N-[(R)-2-Hydroxy-4-methyl-1-oxopentyl]-L-Pro-L-Ile-N-methyl-L-Val-N-methyl-L-Ala-]-β-alanine lactone
Cyclo[N-methyl-L-alanyl-β-alanyl-(2R)-2-hydroxy-4-methylpentanoyl-L-prolyl-L-isoleucyl-N-methyl-L-valyl]
N-[N-[(2R)-1-Oxo-2-hydroxy-4-methylpentyl]-L-Pro-L-Ile-N-methyl-L-Val-N-methyl-L-Ala-]-β-alanine lactone

物理化学性质

熔点234 °C

沸点875.0±65.0 °C(Predicted)

密度1.17±0.1 g/cm3(Predicted)

储存条件-20°C

溶解度溶于DMSO（高达10mg/ml）

酸度系数(pKa)13.54±0.70(Predicted)

形态固体

颜色白色

稳定性可在-20°C下的DMSO溶液保存长达1个月。

安全数据

海关编码2933790090

绿僵菌素B, 来源于绿僵菌属价格(试剂级)

报价日期	产品编号	产品名称	CAS号	包装	价格
2024/04/30	HY-N6690	绿僵菌素B, 来源于绿僵菌属 Destruxin B	2503-26-6	1mg	7800元
2023/10/26	HY-N6690	绿僵菌素B, 来源于绿僵菌属 Destruxin B	2503-26-6	5mg	27500元

常见问题列表

生物活性

Destruxin B 是从昆虫病原真菌中分离的绿僵菌 (Metarhizium anisopliae)，是一种具有杀虫和抗癌活性的环肽。 Destruxin B 通过 Bcl-2 家族依赖的线粒体途径在人类非小细胞肺癌细胞中诱导细胞凋亡。Destruxin B 显著激活 caspase-3，并在体外和体内通过 caspase 介导的细胞凋亡减少肿瘤细胞增殖。

靶点

Bcl-2

Caspase-3

体外研究

Destruxin B (1-30 μM; 48 hours) inhibits cell proliferation of H1299 cells and A549 cells, with IC ₅₀ s of 4.1 μM and 4.9 μM, respectively.
Activation of mitochondria-dependent caspase cascade plays an important role in Destruxin B (1-30 μM; 48 hours) induced apoptotic cell death in A549 cells.
Destruxin B (1.25-20.00 μM; 72 hours) treatment significantly inhibits HT-29 human colorectal cancer cells viability in time- and dose-dependent manners.
Destruxin B (10 or 20 μM; 12 and 24 hours) treatment markedly increases levels of the proapoptotic protein PUMA and reduces levels of antiapoptotic proteins Mcl-1 of A549 cells in a concentration- and time-dependent manner .

Cell Proliferation Assay

Cell Line:	H1299 cells
Concentration:	1, 5, 10, 20, 30 μM
Incubation Time:	48 hours
Result:	Inhibited H1299 cells proliferation of H1299 cells with an IC ₅₀ of 4.1 μM.

Apoptosis Analysis

Cell Line:	Lung adenocarcinoma A549 cells
Concentration:	1, 5, 10, 20, 30 μM
Incubation Time:	48 hours
Result:	Induces caspase-dependent Lung adenocarcinoma A549 cells death.

Cell Viability Assay

Cell Line:	HT-29 human colorectal cancer cells
Concentration:	1.25, 2.50, 5.00, 10.00 and 20.00 μM
Incubation Time:	72 hours
Result:	The IC ₅₀ s at 24 h, 48 h and 72h were determined as 14.97, 2.00 and 0.67 μM, respectively.

Western Blot Analysis

Cell Line:	A549 cells
Concentration:	10 or 20 μM
Incubation Time:	12 and 24 hours
Result:	Regulates Mcl-1 and PUMA.

体内研究

Destruxin B (DB) ( injection; 0.6-15 mg/kg/day for 6 weeks) attenuates the tumor growth in time- and dose-dependent manners.

Animal Model:	Athymic female nude mice (BALB/cAnN.Cg-Foxn1nu/CrlNarl), approximately 4-5 weeks old on arrival
Dosage:	Low dose (0.6 mg/kg), medium dose (3 mg/kg), high dose (15 mg/kg)
Administration:	Injection; daily; for 6 weeks
Result:	Low dose, medium dose and high dose indicated a reduction of 23.9%, 33.4% and 55.8% in mean tumor size, respectively.