PIMONIDAZOLE
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- CAS号:
- 70132-51-3
- 英文名:
- pimonidazole
- 英文别名:
- PIMONIDAZOLE HCL;inhibit,Pimonidazole hydrochloride,Pimonidazole,Inhibitor
- 中文名:
- PIMONIDAZOLE
- 中文别名:
- 化合物 T16536;化合物PIMONIDAZOLE HYDROCHLORIDE
- CBNumber:
- CB41370117
- 分子式:
- C11H19ClN4O3
- 分子量:
- 290.74656
- MOL File:
- 70132-51-3.mol
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PIMONIDAZOLE化学性质
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储存条件:
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Store at -20°C
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溶解度:
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Water: 5 mg/mL (17.20 mM; ultrasonic and warming and heat to 80°C)
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PIMONIDAZOLE性质、用途与生产工艺
Pimonidazole 是一种缺氧标记物,用于瘤内缺氧和细胞增殖的补充性研究。Pimonidazole 通过与大分子的共价结合或通过被还原形成还原性代谢物后在缺氧细胞中积累,可用于定性和定量评估肿瘤缺氧。
Pimonidazole, the exogenous hypoxia marker, is a 2-nitroimidazole compound, which forms covalent bonds with cellular macromolecules at oxygen levels below 1.3%.
Detection
: Hypoxic cells were recognized by immunohistochemical detection of pimonidazole using a mouse monoclonal antibody. Cell proliferation was detected with a commercially available monoclonal antibody for proliferating cell nuclear antigen (PCNA). Assessment of hypoxia and cell proliferation was made qualitatively with light microscopy and quantitatively using point counting and image analysis software methods.
The pimonidazole-protein adducts were then detected by immunofluorescence
Cells (90% confluent) are treated with pimonidazole hydrochloride (10-100 μM), diluted in complete media or PBS, and placed in hypoxia or normoxia for 2-4 hours.Then, cells are washed 4 times in HBSS, fixed for 10 min with 10% neutral buffered formalin at room temperature, washed 3 times with tris buffered saline, and mounted with Prolong Gold with DAPI mounting media.To monitor the intrinsic hypoxic signal, the tissue is removed and the embryos incubated with 400 µM pimonidazole for 2 h before fixation. Immunostaining is performed using antibodies against pimonidazole-protein adducts (Hypoxyprobe).
Pimonidazole is a 2-nitroimidazole that is reductively activated specifically in hypoxic cells and forms stable adducts with thiol groups in proteins, peptides, and amino acids. Additionally, the amount of pimonidazole that is detected is directly proportional to the level of hypoxia within tumors in vivo.
The following protocol is provided for this useful tool to interrogate the levels of hypoxia
in vivo
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1.Pimonidazole hydrochloride is suspended at a concentration of 30 mg/ml in 0.9% sterile saline or PBS.
2.Multiple mice are injected intravenously (tail vein) with 60 mg/kg of the pimonidazole solution.
3.Let circulate in vivo for 90 min before the mice are euthanazied with isoflurane or carbon dioxide (CO2).
Note: For animal euthanasia: In compliance with proper IACUC protocols!
4. Mice are sacrificed and organs are then removed, weighed, snap-frozen, and stored in plastic scintillation vials at -80 °C.
5. The pimonidazole stain can work on paraffin-embedded tissue, frozen tissue, and cell lines. Organs are embedded in OCT, placed in a cryostat, and cut into 10 μm-thick sections. Sections are placed on microscope slides for staining.
PIMONIDAZOLE
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70132-51-3, PIMONIDAZOLE 相关搜索:
- 化合物PIMONIDAZOLE HYDROCHLORIDE
- 化合物 T16536
- 70132-51-3
- inhibit,Pimonidazole hydrochloride,Pimonidazole,Inhibitor
- PIMONIDAZOLE HCL