ChemicalBook >> CAS DataBase List >>DAF-FM DA

DAF-FM DA

CAS No.
254109-22-3
Chemical Name:
DAF-FM DA
Synonyms
DAF-FM DA;DAF-FM DA solution;-[9H]xanthen]-3-one;DAF-FM DA (cell permeable);DAF-FM DA [DAF-FM Diacetate];-difluoro-spiro[isobenzofuran-1(3H);Diaminofluorescein-FM diacetate;DAF-FM Diacetate NO fluorescent probe;DAF-FM DA - CAS 254109-22-3 - Calbiochem;4-Amino-5-(N-methylamin)-3′,6′-bis(acetyloxy)
CBNumber:
CB21074275
Molecular Formula:
C25H18F2N2O7
Molecular Weight:
496.42
MDL Number:
MFCD16872050
MOL File:
254109-22-3.mol
MSDS File:
SDS
Last updated:2023-06-30 15:45:59

DAF-FM DA Properties

Boiling point 695.7±55.0 °C(Predicted)
Density 1.56±0.1 g/cm3(Predicted)
storage temp. -20°C
solubility Soluble in dimethyl sulfoxide
pka 4.20 ± 0.20, most basic, temperature: 25 °C
form Pale yellow solution
color clear clear, colorless
λmax 365 nm (MeOH)
Biological Applications Detecting bacteria/microorganisms; nitric oxide indicator,

SAFETY

Risk and Safety Statements

WGK Germany  3

DAF-FM DA price More Price(13)

Manufacturer Product number Product description CAS number Packaging Price Updated Buy
Sigma-Aldrich D1946 DAF-FM DA ≥98% (HPLC) 254109-22-3 1mg $737 2024-03-01 Buy
Sigma-Aldrich 251520-M DAF-FM DA - CAS 254109-22-3 - Calbiochem A cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~3 nM. 254109-22-3 251520-1MG $1010 2024-03-01 Buy
Sigma-Aldrich 251520-M DAF-FM DA - CAS 254109-22-3 - Calbiochem A cell-permeable, photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~3 nM. 254109-22-3 1mg $957 2023-01-07 Buy
Cayman Chemical 18767 DAF-FM diacetate ≥98% 254109-22-3 1mg $413 2024-03-01 Buy
Cayman Chemical 18767 DAF-FM diacetate 254109-22-3 5mg $1543 2024-03-01 Buy
Product number Packaging Price Buy
D1946 1mg $737 Buy
251520-M 251520-1MG $1010 Buy
251520-M 1mg $957 Buy
18767 1mg $413 Buy
18767 5mg $1543 Buy

DAF-FM DA Chemical Properties,Uses,Production

Uses

DAF-FM diacetate is an important reagent for quantification of low concentrations of nitric oxide in solution. This compound is essentially nonfluorescent until it reacts with NO to form a fluorescent benzotriazole. With excitation/emission maxima of 495

Uses

DAF-FM DA has been used to measure the nitric oxide (NO) levels in liver tissue homogenates. It has also been used in NO staining for detection of NO from human umbilical vein endothelial cells (HUVECs).

Biochem/physiol Actions

DAF-FM DA is a cell-permeable fluorescent probe for the detection of nitric oxide (NO). DAF-2 is the original compound and is widely used, but DAF-FM and DAF-FM DA are more sensitive to nitric oxide (NO) compared with DAF-2 and more photo-stable and less pH sensitive. DAF-FM DA permeates well into living cells and is rapidly transformed into water-soluble DAF-FM by cytosolic esterases.

storage

Store at -20°C

DAF-FM DA Preparation Products And Raw materials

Raw materials

Preparation Products

DAF-FM DA Suppliers

Global( 70)Suppliers
Supplier Tel Email Country ProdList Advantage
Chongqing Chemdad Co., Ltd
+86-023-6139-8061 +86-86-13650506873 sales@chemdad.com China 39916 58
Zhengzhou Alfa Chemical Co.,Ltd
+8618530059196 sale04@alfachem.cn China 12822 58
TargetMol Chemicals Inc.
+1-781-999-5354 +1-00000000000 marketing@targetmol.com United States 19892 58
Career Henan Chemica Co
+86-0371-86658258 15093356674; laboratory@coreychem.com China 30255 58
Alfa Chemistry
+1-5166625404 Info@alfa-chemistry.com United States 21317 58
LEAPCHEM CO., LTD.
+86-852-30606658 market18@leapchem.com China 43348 58
Aladdin Scientific
+1-+1(833)-552-7181 sales@aladdinsci.com United States 57511 58
Dalian Meilun Biotech Co., Ltd. 0411-62910999 13889544652 sales@meilune.com China 4663 58
Artis Chemistry (Shanghai) Co. Ltd. 86-21-60936353 China 336 58
Haoyuan Chemexpress Co., Ltd. 021-58950125 info@chemexpress.com China 7553 61
4-Amino-5-(N-methylamin)-3′,6′-bis(acetyloxy) 4-Amino-5-(N-methylamino)-3′,6′-bis(acetyloxy)-2′,7′-difluoro-spiro[isobenzofuran-1(3H),9′-[9H]xanthen]-3-one DAF-FM DA solution DAF-FM DA (cell permeable) -[9H]xanthen]-3-one Diaminofluorescein-FM diacetate -difluoro-spiro[isobenzofuran-1(3H) DAF-FM DA DAF-FM DA - CAS 254109-22-3 - Calbiochem 4-Amino-2',7'-Difluoro-5-(Methylamino)-3-Oxo-3H-Spiro[2-Benzofuran-1,9'-Xanthene]-3',6'-Diyl Diacetate DAF-FM DA [DAF-FM Diacetate] 3-Amino-4-(N-methylamino)-2', 7'-difluorofluorescein diacetate Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 3',6'-bis(acetyloxy)-4-amino-2',7'-difluoro-5-(methylamino)- DAF-FM Diacetate NO fluorescent probe 6-acetyloxy-4-amino-2,7-difluoro-5-(methylamino)-3-oxospiro2-benzofuran-1,9-xanthene-3-yl acetate 254109-22-3