| Company Name: |
Sigma-Aldrich
|
| Tel: |
021-61415566 800-8193336 |
| Email: |
orderCN@merckgroup.com |
| Products Intro: |
Product Name:Fluorescein-12-dUTP
Purity:Fluorescein-12-dUTP Package:1EA Remarks:11373242910
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| Company Name: |
BOC Sciences
|
| Tel: |
16314854226 |
| Email: |
inquiry@bocsci.com |
| Products Intro: |
Product Name:Fluorescein-12-dUTP
Purity:95% by HPLC
|
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| | FLUORESCEIN-12-DUTP Basic information |
| Product Name: | FLUORESCEIN-12-DUTP | | Synonyms: | CHROMATIDE(TM) FLUORESCEIN-12-DUTP;FLUORESCEIN-12-DUTP;FLUOR-12-DUTP;FLUORESCEIN-6-CARBOXAMINOCAPROYL-[5-[3-AMINOALLYL]-2'-DEOXYURIDINE-5'-TRIPHOSPHATE] | | CAS: | | | MF: | C38H41N4O19P3 | | MW: | 950.67 | | EINECS: | | | Product Categories: | | | Mol File: | Mol File |  |
| | FLUORESCEIN-12-DUTP Chemical Properties |
| storage temp. | -20°C | | form | solution |
| | FLUORESCEIN-12-DUTP Usage And Synthesis |
| Uses | Fluorescein-12-dUTP is used as a substrate for:
- terminal transferase
- DNA polymerase I (holoenzyme and Klenow fragment),
- Taq DNA polymerase
- reverse transcriptase (e.g., from AMV and M-MuLV).
Fluorescein-12-dUTP has been used to label nuclear extracts during PCR. It has also been used to replace dTTP in the random-primed DNA labeling reaction or in nick translation reactions, as well as in PCR. The nucleotide also serves as a substrate for terminal transferase in 3′-end labeling and TUNEL. Fluorescein-labeled probes can be used for in in situ hybridization with direct fluorescence detection or detection by enzyme linked immunoimmunsorbent assay (ELISA) using Anti-Fluorescein-AP, Fab fragments. Repeated fluorescence labeling using Tetramethyl-Rhodamine-6-dUTP (red) and AMCA-6-dUTP (bright blue) is possible. |
| | FLUORESCEIN-12-DUTP Preparation Products And Raw materials |
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