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| | Propanamide, 2-(acetylamino)-3-mercapto-N-methyl-, (2R)- Basic information |
| | Propanamide, 2-(acetylamino)-3-mercapto-N-methyl-, (2R)- Chemical Properties |
| Melting point | 196-197 °C | | Boiling point | 454.7±40.0 °C(Predicted) | | density | 1.145±0.06 g/cm3(Predicted) | | solubility | Soluble to 100 mM in DMSO and to 100 mM in water | | pka | 9.06±0.10(Predicted) |
| | Propanamide, 2-(acetylamino)-3-mercapto-N-methyl-, (2R)- Usage And Synthesis |
| Biological Activity | Ac-Cys-NHMe is a non-canonical amino acid that is an essential building block for lysine acylation using conjugating enzymes (LACE) technique. LACE is a chemoenzymatic approach that uses an E2 small ubiquitin-like modifier (SUMO)-conjugating enzyme, Ubc9, to conjugate peptide thioesters containing a C-terminal ubiquitin-derived sequence (LRLRGG) to a lysine residue located in a recognition sequence (IKQE) on a protein via an isopeptide bond. Ac-Cys-NHMe can be reacted with a peptide hydrazide to form a reactive peptide thioester, which is the substrate for Ubc9. LACE enables the site-specific modification of proteins at lysine residues to allow attachment of biochemical probes such as fluorophores to proteins or to engineer proteins for site-specific delivery. | | storage | Store at -20°C |
| | Propanamide, 2-(acetylamino)-3-mercapto-N-methyl-, (2R)- Preparation Products And Raw materials |
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