Cytidine 5’-monophosphate synthesis
- Product Name:Cytidine 5’-monophosphate
- CAS Number:63-37-6
- Molecular formula:C9H14N3O8P
- Molecular Weight:323.2
3616-08-8
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63-37-6
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Yield:-
Reaction Conditions:
with bovine serum albumin in aq. buffer at 30;Enzymatic reaction;
Steps:
2.3. Time course of PDE7A activity
General procedure: Time course experiments were performed in PDE buffer + 0.05% (m/v) BSA at a cAMP- or cCMP-concentration of 3 μM. The purified GST-tagged and truncated PDE7A1/2 was added at a concentration of 0.95 μg/ml, corresponding to 210 pmol/(min ml). Crude PDE7A1-containing Sf9 cell lysate was added to yield a final activity of 5 U/ml. The samples were incubated at 30 °C under constant shaking. Aliquots were drawn at appropriate times, processed as described for the enzyme screening experiments (Section 2.2) and analyzed by HPLC-MS/MS as described in the Supplementary Methods. In all experiments, the samples were repeatedly subjected to short centrifugations (at least every 30 min) to avoid concentration changes by evaporation- and condensation processes.
References:
Monzel, Maike;Kuhn, Maike;B?hre, Heike;Seifert, Roland;Schneider, Erich H. [FEBS Letters,2014,vol. 588,# 18,p. 3469 - 3474]
362-42-5
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63-37-6
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65-46-3
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63-37-6
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149759-93-3
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63-37-6
542 suppliers
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78571-59-2
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63-37-6
542 suppliers
$12.00/25g