| Identification | Back Directory | [Name]
DIHYDROETHIDIUM | [CAS]
104821-25-2 | [Synonyms]
PD-MY 003
Hydroethidine,98%
HYDROETHIDINE™
DIHYDROCYCLOSPORINH
(DIHYDROETHIDIUM BROMIDE)
DIHYDROETHIDIUM, FOR FLUORESCENCE*
hydroethidine(dihydroethidiumbromide)
5-ethyl-6-phenyl-5,6-dihydrophenanthridine-3,8-diaMine
3,8-Phenanthridinediamine,5-ethyl-5,6-dihydro-6-phenyl-
2,7-Diamino-10-ethyl-9-phenyl-9,10-dihydrophenanthridine 3,8-Diamino-5,6-dihydro-5-ethyl-6-phenylphenanthridine Hydroethidine | [Molecular Formula]
C21H20BrN3 | [MDL Number]
MFCD00077335 | [MOL File]
104821-25-2.mol | [Molecular Weight]
394.308 |
| Chemical Properties | Back Directory | [Appearance]
pink to purple fine crystalline powder | [Boiling point ]
580.4±50.0 °C(Predicted) | [density ]
1?+-.0.06 g/cm3(Predicted) | [storage temp. ]
−20°C
| [solubility ]
acetonitrile: soluble
| [form ]
powder | [pka]
5.30±0.40(Predicted) | [color ]
Pink to dark brown | [λmax]
355 nm | [BRN ]
5107482 | [Biological Applications]
Apoptosis assay; generating and detecting reactive oxygen species (ROS); detecting nucleic acids,cells; measuring respiratory burst; superoxide indicator; viability assay | [InChI]
InChI=1S/C21H21N3/c1-2-24-20-13-16(23)9-11-18(20)17-10-8-15(22)12-19(17)21(24)14-6-4-3-5-7-14/h3-13,21H,2,22-23H2,1H3 | [InChIKey]
XYJODUBPWNZLML-UHFFFAOYSA-N | [SMILES]
C1=C2C(N(CC)C(C3=CC=CC=C3)C3=C2C=CC(N)=C3)=CC(N)=C1 |
| Hazard Information | Back Directory | [Chemical Properties]
pink to purple fine crystalline powder | [Uses]
Redox indicator. Blue fluorescence until oxidized to ethidium. Can be used for detecting superoxide radical in cells, tissues and organisms. | [Description]
Dihydroethidium is a cell-permeable blue fluorescent dye, which intercalates with nucleic acids and emits a red fluorescence detectable qualitatively by fluorescent microscopy or quantitatively by HPLC. It is a superoxide indicator. It exhibits blue fluorescence in the cytosol until oxidizedto ethidium, where it intercalates within the cell's DNA, staining its nucleus a bright fluorescent red. It is neuroprotective by reducing superoxide in mice after stroke. It has been used to detect reactive oxygen species during the phagocytic respiratory burst and for the detection of intracellular superoxide in cultured cells. | [Excitation / Emission Maximum]
490 nm/590 nm |
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