| Identification | Back Directory | [Name]
7-AMINO-4-METHYL-3-COUMARINYLACETIC ACID | [CAS]
106562-32-7 | [Synonyms]
AMCA-H
AMCA blue
AMCA acid
AMCA (coumarin)
2H-1-Benzopyran-3-acetic acid
AMCA acid *CAS#: 106562-32-7*
AMINO-METHYL-COUMARINACETICACID
7-AMINO-4-METHYL-3-COUMARINYLACETIC ACID
7-Amino-4-methylcoumarin-3-aceticAcid>
FMOC-7-AMINO-4-METHYL-3-COUMARINYLACETIC ACID
7-Amino-4-methyl-3-coumarinylacetic acid(AMCA)
2-(7-amino-4-methyl-2-oxochromen-3-yl)acetic acid
AMCA-H [7-AMino-4-Methyl-3-couMarinylacetic acid ]
7-AMINO-4-METHYL-2-OXO-2H-CHROMEN-3-YL ACETIC ACID
2-(7-aMino-4-Methyl-2-oxo-2H-chroMen-3-yl)acetic acid
2H-1-Benzopyran-3-acetic acid, 7-amino-4-methyl-2-oxo-
7-Amino-4-methyl-2-oxo-2H-chromen-3-yl acetic acid, 90+%
7-AMINO-4-METHYL-3-COUMARINYLACETIC ACID BIOCHEMIKA, FOR FLUORESCENCE 90% | [Molecular Formula]
C12H11NO4 | [MDL Number]
MFCD00081051 | [MOL File]
106562-32-7.mol | [Molecular Weight]
233.22 |
| Chemical Properties | Back Directory | [Boiling point ]
512.2±50.0 °C(Predicted) | [density ]
1.391±0.06 g/cm3 (20 ºC 760 Torr) | [storage temp. ]
Keep in dark place,Sealed in dry,Room Temperature | [solubility ]
DMF: soluble | [form ]
powder to crystal | [pka]
3.80±0.10(Predicted) | [color ]
Light yellow to Brown | [Appearance]
Solid | [λmax]
344nm(Buffer)(lit.) | [BRN ]
7927205 | [InChI]
InChI=1S/C12H11NO4/c1-6-8-3-2-7(13)4-10(8)17-12(16)9(6)5-11(14)15/h2-4H,5,13H2,1H3,(H,14,15) | [InChIKey]
QEQDLKUMPUDNPG-UHFFFAOYSA-N | [SMILES]
C1(=O)OC2=CC(N)=CC=C2C(C)=C1CC(O)=O |
| Safety Data | Back Directory | [Hazard Codes ]
Xi | [Risk Statements ]
36/37/38 | [Safety Statements ]
26-36 | [WGK Germany ]
3
| [HS Code ]
29322090 | [Storage Class]
11 - Combustible Solids | [Hazard Classifications]
Eye Irrit. 2
Skin Irrit. 2
STOT SE 3 |
| Hazard Information | Back Directory | [Description]
AMCA is fluorescent protein labeling agent.1 It contains an N-hydroxysuccinimide ester that reacts with lysine residues to form photostable amide links. Upon activation with UV light, AMCA displays emission maxima of 400-460 nm. It has commonly been used in multiplex immunophenotyping applications.2,3,4 | [Uses]
7-AMINO-4-METHYL-3-COUMARINYLACETIC ACID is a blue fluorescent dye. Its desirable properties include a relatively large Stoke's shift and resistance to photobleaching. 7-AMINO-4-METHYL-3-COUMARINYLACETIC ACID is used as contrasting probes for double and triple labeling in im
| [Uses]
Non-Activated, Amine-Reactive, fluorescent probe | [Chemical Reactivity]
Primary amines (needs activation) | [References]
[1] H. KHALFAN. Aminomethyl coumarin acetic acid: A new fluorescent labelling agent for proteins[J]. Journal of Molecular Histology, 1986, 18 9: 497-499. DOI: 10.1007/bf01675617
[2] G L FERRI. Quadruple immunofluorescence: a direct visualization method.[J]. Journal of Histochemistry & Cytochemistry, 1997, 45 2: 155-158. DOI: 10.1177/002215549704500201
[3] P. M. NEDERLOF. Three-color fluorescence in situ hybridization for the simultaneous detection of multiple nucleic acid sequences[J]. Cytometry Part B: Clinical Cytometry, 1989, 10 1: 20-27. DOI: 10.1002/cyto.990100105
[4] ANDRé GOTHOT Jean M P Jean Claude Grosdent. A strategy for multiple immunophenotyping by image cytometry: Model studies using latex microbeads labeled with seven streptavidin-bound fluorochromes[J]. Cytometry Part A, 1996, 24 3: 214-225. DOI: 10.1002/(sici)1097-0320(19960701)24:3<214::aid-cyto4>3.0.co;2-h | [Abs/Em Maxima]
345/450 nm | [Extinction Coefficient]
19,000 cm-1M-1 | [Spectrally Similar Dyes]
Alexa Fluor® 350, AMCA, DyLight® 350 |
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