| Identification | Back Directory | [Name]
TETRAMETHYLRHODAMINE METHYL ESTER PERCHLORATE | [CAS]
115532-50-8 | [Synonyms]
TMRM
T 668
TMRM, PERCHLORATE
Tetramethylrhodamin methylester perchlorate
TETRAMETHYLRHODAMINE METHYL ESTER PERCHLORATE
TMRM [TetraMethylrhodaMine, Methyl ester, perchlorate]
TMRM [Tetramethylrhodamine methyl ester] *CAS#: 115532-50-8*
3,6-bis(diMethylaMino)-9-(2-(Methoxycarbonyl)phenyl)xanthyliuM perchlorate
XanthyliuM, 3,6-bis(diMethylaMino)-9-[2-(Methoxycarbonyl)phenyl]-, perchlorate (1:1)
N-[6-(Dimethylamino)-9-[2-(methoxycarbonyl)phenyl]-3H-xanthen-3-ylidene]-N-methylmethanaminium Perchlorate | [Molecular Formula]
C25H25ClN2O7 | [MDL Number]
MFCD00274460 | [MOL File]
115532-50-8.mol | [Molecular Weight]
500.93 |
| Chemical Properties | Back Directory | [Melting point ]
274–276℃ | [storage temp. ]
2-8°C
| [solubility ]
DMSO: soluble
| [form ]
crystals | [color ]
Dark green | [Appearance]
Solid Powder | [λmax]
549 nm | [BRN ]
7162165 | [Biological Applications]
Detecting mitochondrial membrane potential; apoptosis assays; multidrug resistance assays |
| Hazard Information | Back Directory | [Description]
Tetramethylrhodamine methyl ester (TMRM) perchlorate is a cell-permeant red fluorescent probe commonly used to monitor membrane potential of mitochondria using live cell fluorescence microscopy and flow cytometry. TMRM is a lipophilic cation accumulated by mitochondria and exhibits a red shift in the emission of 575 nm. However, when the membrane is depolarized, as in apoptosis, TMRM is not accumulated in the mitochondria. Thus, the strength of the fluorescence signal in mitochondria is used to assess cell viability. | [Uses]
Potential-Sensitive fluorescent probe for measuring membrane potential changes in mitochondria | [Excitation / Emission Maximum]
515, 555 nm/575 nm |
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