| Chemical Properties | Back Directory | [storage temp. ]
Store at -20°C | [solubility ]
Soluble to 1 mg/ml in PBS (pH 7.4) | [form ]
Powder | [color ]
Light yellow to yellow | [Sequence]
{4-((N′-2-methylphenyl)ureido)-phenylacetyl}-Leu-Asp-Val-Pro-Ala-Ala-{Lys(FITC)} |
| Hazard Information | Back Directory | [Description]
Fluorescent ligand that binds to theα4β1integrin (VLA-4) with high affinity (Kdvalues are 0.3 nM and 12 nM for binding to U937 cells in the presence and absence of Mn2+respectively). Used to detect VLA-4 affinity and conformational changes. | [Uses]
LDV-FITC, a fluorescent peptide, is a FITC-conjugated LDV peptide (HY-P2267). LDV-FITC binds to the α4β1 integrin with high affinity (Kd: 0.3 nM and 12 nM for binding to U937 cells in the presence and absence of Mn2+ respectively). LDV-FITC can be used to detect α4β1 integrin affinity[1][2]. | [storage]
Store at -20°C | [References]
[1] Hyduk SJ, et al. Phospholipase C, calcium, and calmodulin are critical for alpha4beta1 integrin affinity up-regulation and monocyte arrest triggered by chemoattractants. Blood. 2007 Jan 1;109(1):176-84. DOI:10.1182/blood-2006-01-029199
[2] Chigaev A, et al. Real time analysis of the affinity regulation of alpha 4-integrin. The physiologically activated receptor is intermediate in affinity between resting and Mn(2+) or antibody activation. J Biol Chem. 2001 Dec 28;276(52):48670-8. DOI:10.1074/jbc.M103194200 |
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