ChemicalBook--->CAS DataBase List--->1221498-88-9

1221498-88-9

1221498-88-9 Structure

1221498-88-9 Structure
IdentificationBack Directory
[Name]

BIOTIN-11-DUTP
[CAS]

1221498-88-9
[Synonyms]

BIOTIN-11-DUTP
6-[[5-[(3aS,4S,6aR)-2-oxido-3a,4,6,6a-tetrahydro-1H-thieno[3,4-d]imidazol-4-yl]-1-oxidopentylidene]amino]-N-[(E)-3-[4-amino-1-[4-hydroxy-5-[[hydroxy-[hydroxy(phosphonooxy)phosphoryl]oxyphosphoryl]oxymethyl]oxolan-2-yl]-2-oxido-4H-pyrimidin-5-yl]prop-2-enyl]hexanimidate
[Molecular Formula]

C28H46LiN6O17P3S
[MDL Number]

MFCD02180971
[MOL File]

1221498-88-9.mol
[Molecular Weight]

870.62
Chemical PropertiesBack Directory
[solubility ]

soluble in water
[Appearance]

colorless solid
Hazard InformationBack Directory
[Description]

Biotinylated deoxyuridine triphosphate (dUTP) can be used for DNA labeling using various methods, including Nick-translation and 3’-terminal non-radioactive labeling. Generated probes can be used in various hybridization experiments such as Southern blot, Northern blot, dot blot, or FISH. Biotin-labeled probes are detected with horseradish peroxidase-conjugated streptavidin or other biomolecule conjugates binding biotin.


«11» in the compound name indicates the length of the linker between dUTP and biotin; this linker increases the efficiency of labeled dUTP incorporation into DNA and interaction of biotin with specific proteins such as avidin/streptavidin or anti-biotin antibodies.

[Uses]

Biotin-11-dCTP is a biotin (B389040) labelled nucleotide that is used for non-fluorescent labelling of nucleic acids in purification and detection applications.
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