| Identification | Back Directory | [Name]
5-CFDA, AM | [CAS]
124412-00-6 | [Synonyms]
CFDA-AM
5-CFDA, AM
YDBUJZYYYBVSEF-UHFFFAOYSA-N
5-CFDA-AM, 90%, single isomer
CFDAAMCarboxyfluoresceindiacetateAMester
5-CARBOXYFLUORESCEIN DIACETATE, ACETOXYMETHYL ESTER
5-Carboxyfluorescein diacetate acetoxylmethyl ester
5-Carboxyfluorescein diacetate (acetyloxy)methyl ester
5-(AND-6)-CARBOXY SNARF(R)-1, ACETOXYMETHYL ESTER, ACETATE
5-CFDA-AM [5-carboxyfluorescein diacetate, acetoxymethyl ester]
3',6'-Bis(acetyloxy)-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-5-carboxylic acid (acetyloxy)methyl ester
Spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-5-carboxylic acid, 3',6'-bis(acetyloxy)-3-oxo-, (acetyloxy)methyl ester | [Molecular Formula]
C28H20O11 | [MDL Number]
MFCD00467519 | [MOL File]
124412-00-6.mol | [Molecular Weight]
532.45 |
| Chemical Properties | Back Directory | [Boiling point ]
737.0±60.0 °C(Predicted) | [density ]
1.51±0.1 g/cm3(Predicted) | [form ]
Solid | [color ]
White to off-white | [Appearance]
Solid Powder |
| Hazard Information | Back Directory | [Description]
5-carboxyfluorescein diacetate acetoxymethyl ester is a cell-permeant fluorogenic esterase substrate. It can be converted by nonspecific esterases of living cells from a membrane-permeable, nonpolar, nonfluorescent substance to a polar product CF, indicating the integrity of the plasma membrane. | [Uses]
5-CFDA-AM is a cell-permeable esterase substrate that can be used as an active probe to measure enzyme activity and cell membrane integrity. 5-CFDA-AM is electroneutral and can enter the cell at a lower concentration than CFDA, where it is hydrolysed by intracellular esterases to produce carboxyfluorescein. Carboxyfluorescein contains an additional negative charge and can be better retained in the cell[1]. | [References]
[1] Elena Gorokhova, et al. A comparison of TO-PRO-1 iodide and 5-CFDA-AM staining methods for assessing viability of planktonic algae with epifluorescence microscopy. J Microbiol Methods. 2012 Jun;89(3):216-21. DOI:10.1016/j.mimet.2012.03.005 |
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