| Identification | Back Directory | [Name]
ARACHIDONOYL THIO-PC | [CAS]
146797-82-2 | [Synonyms]
Thiopc
ARACHIDONOYL THIO-PC
YDSCLGYBUIDZNF-WWBBCYQPSA-N
2-DEOXY-2-THIO ARACHIDONOYL PC
Arachidonoyl thio-PC (substrate)
2-DEOXY-2-THIOARACHIDONOYLPHOSPHATIDYLCHOLINE
1-hexadecyl-2-arachidonoylthio-2-deoxyglycero-3-phosphorylcholine
1-Hexadecyl-2-arachidonoylthio-2-deoxy-sn-glycero-3-phosphorylcholine
1-O-HEXADECYL-2-DEOXY-2-THIO-R-(5Z,8Z,11Z,14Z-EICOSATETRAENOYL)-SN-GLYCERYL-3-PHOSPHORYLCHOLINE | [Molecular Formula]
C44H82NO6PS | [MDL Number]
MFCD00797668 | [MOL File]
146797-82-2.mol | [Molecular Weight]
784.16 |
| Chemical Properties | Back Directory | [storage temp. ]
Store at -20°C | [solubility ]
DMF: 25 mg/ml; DMSO: 25 mg/ml; Water: 0.5 mg/ml | [form ]
Liquid | [pka]
1.184±0.50(predicted) | [color ]
Colorless to light yellow |
| Hazard Information | Back Directory | [Description]
Arachidonoyl thio-PC is a substrate for many phospholipase A2s (PLA2s) including sPLA2, cPLA2, and iPLA2. Cleavage of the sn-2 fatty acid by PLA2 results in generation of a free thiol which reacts with chromogenic reagents such as DTNB (Ellman’s reagent) and DTP to allow quantitation of PLA2 activity. Isozyme-specific cPLA2 activity can be measured by excluding or inhibiting sPLA2 and iPLA2 activities in the assay. | [Uses]
Arachidonoyl Thio-PC is a substrate of many phospholipase A2 (PLA2), including sPLA2, cPLA2 and iPLA2. Cleavage of sn-2 fatty acids by PLA2 results in the production of free thiols, which react with chromogenic reagents such as DTNB (Ellman's reagent) and DTP, allowing quantification of PLA2 activity. Isozyme-specific cPLA2 activity can be measured by depleting or inhibiting sPLA2 and iPLA2 activity in the assay. | [References]
[1] L YU E A D. Thio-based phospholipase assay.[J]. Methods in enzymology, 1991, 197: 65-75. DOI: 10.1016/0076-6879(91)97133-j |
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