| Identification | Back Directory | [Name]
5(6)-(N-SUCCINIMIDYLOXYCARBONYL)-3',6',O,O'-DIACETYLFLUORESCEIN | [CAS]
150347-59-4 | [Synonyms]
CFSE
CFDA SE
NHS ester
≥90% (HPCE)
5(6)-FAM DA SE
CFSE (CFDA-SE)
-Cellstain-Cfse
Vitamin B12-O5'
5-FAM-NHS DIACETATE
5(6)-CFDA N-succinmidyl ester
5-CARBOXYFLUORESCEIN-NHS DIACETATE
Carboxyfluorescein succinimidyl ester
CARBOXYFLUORESCEIN DIACETATE, NHS ESTER
Cell proliferation tracer fluorescence probe
5(6)-CFDA (SE [5(6)-Carboxyfluorescein diacetate
Carboxyfluorescein diacetate, succinimidyl ester
5-CARBOXY-FLUORESCEIN DIACETATE N-SUCCINIMIDYL ESTER
5-(6)-carboxy' Uorescein Diacetate Succinimidyl Ester
5-CARBOXY-DI-O-ACETYLFLUORESCEIN N-SUCCINIMIDYL ESTER
5(6)-carboxyfluorescein diacetate N-succinimidyl este
5(6)-CARBOXYFLUORESCEIN DIACETATE N-SUCCINIMIDYL ESTER
5-CARBOXYFLUORESCEIN DIACETATE-N-HYDROXYSUCCINIMIDE ESTER
5(6)-Carboxyfluorescein diacetate, succinimidyl ester(CFDA)
5(6)-Carboxyfluorescein 3',6'-Diacetate N-SucciniMidyl Ester
5-or6-(N-Succinimidyloxycarbonyl)-fluorescein3',6'-diacetate
5(6)-CARBOXYFLUORESCEIN DIACETATE-N-HYDROXY-SUCCINIMIDE ESTER
5(6)-(N-SUCCINIMIDYLOXYCARBONYL)-3',6',O,O'-DIACETYLFLUORESCEIN
5-or6-(N-Succiimidyloxycarbonyl)-3',6'-O,O'-diacethylfluorescein
3'',6''-O,O''-Diacetyl-5(6)-(N-succinimidyloxycarbonyl)-fluorescein
5(6)-CFDA, SE [5(6)-Carboxyfluorescein diacetate, succiniMidyl ester]
5-or 6-(N-Succinimidyloxycarbonyl)-3', 6'-O,O'-diacetylfluorescein (CFSE)
1-[[[3',6'-Bis(acetyloxy)-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-
Spiro[isobenzofuran-1(3h),9′-[9h]xanthene], 2,5-pyrrolidinedione derivative
CFSE [5-(and 6)-Carboxyfluorescein diacetate, succinimidyl ester] *CAS 150347-59-4*
ReadiUse CFSE [5-(and 6)-Carboxyfluorescein diacetate, succinimidyl ester] *CAS 150347-59-4*
2,5-Dioxopyrrolidin-1-ylacetatecompoundwith3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-3',6'-diyldiacetate(1:1)
3',6'-Bis(acetyloxy)-3-oxo-spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-ar-carboxylic Acid 2,5-Dioxo-1-pyrrolidinyl Ester
Spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-ar-carboxylicacid, 3',6'-bis(acetyloxy)-3-oxo-, 2,5-dioxo-1-pyrrolidinyl ester | [Molecular Formula]
C29H19NO11 | [MDL Number]
MFCD00037466 | [MOL File]
150347-59-4.mol | [Molecular Weight]
557.46 |
| Chemical Properties | Back Directory | [Melting point ]
152-154 °C(lit.)
| [storage temp. ]
-20°C | [solubility ]
DMF: soluble
| [form ]
Solid | [pka]
6.45(at 25℃) | [color ]
Off-white to yellow | [Appearance]
white solid | [PH Range]
Weak green ' uorescence (6.0) to strong green ' uorescence (7.2) | [λmax]
<300nm | [Major Application]
Diagnosis of cancer, restricting HIV replication, antiinflammatory agent, detecting bacteria, cell proliferation assays | [InChIKey]
QSWYNOQSGMUDQZ-UHFFFAOYSA-N | [SMILES]
C12(OC(=O)C3=CC(C(=O)ON4C(=O)CCC4=O)=CC=C13)C1C=CC(OC(=O)C)=CC=1OC1C=C(OC(=O)C)C=CC2=1 |
| Hazard Information | Back Directory | [Description]
(5,6)-FAM diacetate NHS ester is a cell-permeable amine-reactive label for biomolecules.
This molecule is non-fluorescent until the acetate groups are cleaved by intracellular esterases resulting in the formation of a highly fluorescent fluorophore with emission in the green spectrum range (absorption maximum at ~492 nm, emission maximum at ~517 nm). Due to this characteristic, this product is used for tracking cells and flow cytometric evaluations and studying intracellular esterase. | [Chemical Properties]
5(6)-(N-SUCCINIMIDYLOXYCARBONYL)-3',6',O,O'-DIACETYLFLUORESCEIN is Off-white to light yellow powder
| [Uses]
5(6)-(N-SUCCINIMIDYLOXYCARBONYL)-3',6',O,O'-DIACETYLFLUORESCEIN is a Non-fluorescent cell permeant amine-reactive probe for long term tracing of cell.
| [Uses]
A 5(6)-Carboxyfluorescein (C178215) derivative used as a flurorescent probe. 5(6)-Carboxyfluorescein 3’,6’-Diacetate N-Succinimidyl Ester is used to monitor lymphocyte proliferation by covalently labelling long-lived intracellular molecules with fluorescent dye. | [Uses]
Amine-reactive succinimidyl ester can potentially be used for long-term pH studies of live cells, producing a conjugate with the pH-sensitive properties of carboxyfluorescein. | [General Description]
5-(and-6)-Carboxyfluorescein diacetate N-succinimidyl ester (CFSE) is a fluorescent dye, which is membrane permeable. It labels cells by covalently binding to intracellular molecules. CFSE is used to track lymphocyte migration and proliferation. | [Biochem/physiol Actions]
5(6)-Carboxyfluorescein diacetate N-succinimidyl ester is used as an indicator for esterase activity. | [Abs/Em Maxima]
492/517 nm |
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