| Identification | Back Directory | [Name]
PFM01 | [CAS]
1558598-41-6 | [Synonyms]
PFM01
PFM01 >=98% (HPLC)
(5Z)-5-[(4-Hydroxyphenyl)methylene]-3-(2-methylpropyl)-2-thioxo-4-thiazolidinone | [Molecular Formula]
C14H15NO2S2 | [MDL Number]
MFCD02220972 | [MOL File]
1558598-41-6.mol | [Molecular Weight]
293.4 |
| Chemical Properties | Back Directory | [Boiling point ]
435.2±55.0 °C(Predicted) | [density ]
1.35±0.1 g/cm3(Predicted) | [storage temp. ]
2-8°C | [solubility ]
Soluble in DMSO | [form ]
powder | [pka]
8.59±0.30(Predicted) | [color ]
white to beige | [InChI]
1S/C14H15NO2S2/c1-9(2)8-15-13(17)12(19-14(15)18)7-10-3-5-11(16)6-4-10/h3-7,9,16H,8H2,1-2H3/b12-7- | [InChIKey]
GPURHDUTZUYAFI-GHXNOFRVSA-N | [SMILES]
OC1=CC=C(/C=C2SC(N(CC(C)C)C\2=O)=S)C=C1 |
| Hazard Information | Back Directory | [Uses]
PFM01, N-alkylated Mirin derivative, is a MRE11 endonuclease inhibitor. PFM01 can regulate double-strand break repair (DSBR) by nonhomologous end-joining (NHEJ) versus homologous recombination (HR)[1][2]. | [Biochem/physiol Actions]
PFM01 is a cell-permeable N-alkylated Mirin (Sigma Cat. No. 475954) derivative that selectively inhibits against MRE11 endo-, but not exo-, nuclease activity. PFM01 targets MRE11 at a site near the dimer interface, distinct from that occupied by Mirin and PFM39 to allow disruption of the ssDNA-binding groove and selective inhibition against MRE11 endo-, but not exo-, nuclease activity. While both endonuclease and exonuclease activities are required for MRE11-mediated homologous recombination (HR) repair, only FM01 (100 μM), but not the exonuclease inhibitors Mirin (500 μM) and PFM39 (100 μM), rescues G2-phase double-strand break (DSB) repair defect in HR protein BRCA2-deficient HSC62-hTERT fibroblasts following ionizing irradiation (IR) by blocking HR initiation and thereby allowing non-homologous end joining (NHEJ) to proceed. | [storage]
Store at -20°C | [References]
[1] Shibata A, et, al. DNA double-strand break repair pathway choice is directed by distinct MRE11 nuclease activities. Mol Cell. 2014 Jan 9; 53(1): 7-18. DOI:10.1016/j.molcel.2013.11.003
[2] V?lkening L, et, al. RAD50 regulates mitotic progression independent of DNA repair functions. FASEB J. 2020 Feb; 34(2): 2812-2820. DOI:10.1096/fj.201902318R |
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