| Identification | Back Directory | [Name]
Benzeneacetonitrile, 4-cyano-α-[[4-[(2-hydroxyethyl)methylamino]phenyl]methylene]- | [CAS]
156840-13-0 | [Synonyms]
green,GFP,fluorophore-like,fluorescent,synthetic,HBC,Inhibitor,inhibit,dye
Benzeneacetonitrile, 4-cyano-α-[[4-[(2-hydroxyethyl)methylamino]phenyl]methylene]- | [Molecular Formula]
C19H17N3O | [MDL Number]
MFCD32856424 | [MOL File]
156840-13-0.mol | [Molecular Weight]
303.37 |
| Chemical Properties | Back Directory | [Boiling point ]
538.7±50.0 °C(Predicted) | [density ]
1.22±0.1 g/cm3(Predicted) | [storage temp. ]
Store at -20°C | [solubility ]
DMSO|30.34|100| | [form ]
Solid | [pka]
14.61±0.10(Predicted) | [color ]
Orange to red |
| Hazard Information | Back Directory | [Description]
Mimic of green fluorescent protein (GFP) fluorophore for imaging RNA in live cells. Fluoresces when bound to Pepper aptamer; displays high affinity binding (Kd = 3.5 nM). Suitable for confocal and two-photon microscopy. Confocal excitation maximum = 488 nm, emission maximum= 495 - 595 nm. Two-photon excitation maximum = 976 nm, emission maximum= 475 - 575 nm. To make a 1000x stock solution, add 200 μL DMSO . | [Uses]
HBC (HBC 530) is a GFP fluorophore-like synthetic dye, with a structurally rigid electron acceptor and a strong electron donor. HBC is nonfluorescent in solution, and when combined with Pepper (RNA aptamer), HBC forms a tight complex and activates and emits bright fluorescence (Kd of ~3.5 nM). HBC emission peaks vary in different complexes and covers the spectrum from cyan to red. HBC can be used in the live cell imaging of RNA[1]. | [storage]
Store at -20°C | [References]
[1] Chen X, et al. Visualizing RNA dynamics in live cells with bright and stable fluorescent RNAs. Nat Biotechnol. 2019;37(11):1287-1293. DOI:10.1038/s41587-019-0249-1 |
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