| Hazard Information | Back Directory | [Uses]
Janelia Fluor 646, Tetrazine (JF646, Tetrazine) a red fluorescent dye that contains a tetrazine group. JF646, Tetrazine can be used in cellular imaging[1]. Janelia Fluor products are licensed under U.S. Pat. Nos. 9,933,417, 10,018,624 and 10,161,932 and other patents from Howard Hughes Medical Institute. Janelia Fluor? 646, Tetrazine is a click chemistry reagent, it contains a Tetrazine group that can undergo an inverse electron demand Diels-Alder reaction (iEDDA) with molecules containing TCO groups. | [Biological Activity]
Janelia Fluor? 646, Tetrazine is a red fluorescent dye; supplied with a tetrazine reactive handle for copper-free click chemistry. Suitable for confocal fluorescent imaging, super-resolution microscopy (SRM) techniques such as dSTORM (live and fixed cells) and STED imaging. Can be multiplexed for two-color imaging with Janelia Fluor;® 549 Tetrazine . Also suitable for flow cytometry. Cell-permeable. Excitation maximum = 646 nm; emission maximum = 664 nm. Quantum yield = 0.54, Max. extinction coefficient = 152,000 M-1cm-1 (measured in ethanol plus 0.1% TFA); A280 correction factor is 0.19. | [storage]
Store at -20°C | [References]
[1] Grimm JB, et al. A general method to improve fluorophores for live-cell and single-molecule microscopy. Nat Methods. 2015;12(3):244-250. doi:10.1038/nmeth.3256 DOI:10.1038/nmeth.3256
[2] Basu S, et al. FRET-enhanced photostability allows improved single-molecule tracking of proteins and protein complexes in live mammalian cells. Nat Commun. 2018;9(1):2520. Published 2018 Jun 28. DOI:10.1038/s41467-018-04486-0 |
|
|