| Identification | Back Directory | [Name]
3,3',5,5'-TETRAMETHYLBENZIDINE DIHYDROCHLORIDE HYDRATE, 98+% | [CAS]
207738-08-7 | [Synonyms]
TMB 2HC1.xH2O
TMB*2HCL*2H2O
TMB (hydrochloride hydrate)
3,3',5,5'-tetramethylbenzidine·2HCl·2H2O
3,3μ,5,5μ-Tetramethylbenzidine hydrate dihydrochloride
3,3',5,5'-TetraMethylbenzidine dihydrochloride hydrate 97%
3,3',5,5'-TETRAMETHYLBENZIDINE DIHYDROCHLORIDE HYDRATE, 98+%
3,3',5,5'-Tetramethyl-[1,1'-biphenyl]-4,4'-diamine hydrochloride hydrate
3,3',5,5'-tetraMethylbiphenyl-4,4'-diaMine dihydrochloride hydrate (1:2:)
3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate,TMB dihydrochloride
3,3',5,5'-Tetramethylbenzidine dihydrochloride hydrate Vetec(TM) reagent grade, >=98% | [EINECS(EC#)]
688-511-0 | [Molecular Formula]
C16H20N2.2ClH | [MDL Number]
MFCD00150104 | [MOL File]
207738-08-7.mol | [Molecular Weight]
313.27 |
| Chemical Properties | Back Directory | [Appearance]
Off-white fluffy powder | [Melting point ]
>300°C | [storage temp. ]
2-8°C | [solubility ]
Soluble in DMSO (~1.7 mg/ml), and 1:300 DMSO:PBS (pH 7.2) (0.03 mg/ml). | [form ]
Powder | [color ]
Light brown to brown | [Sensitive ]
Moisture & Light Sensitive | [InChI]
InChI=1S/C16H20N2.ClH.H2O/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14;;/h5-8H,17-18H2,1-4H3;1H;1H2 | [InChIKey]
NYNRGZULARUZCC-UHFFFAOYSA-N | [SMILES]
CC1=C(C(C)=CC(C2C=C(C)C(N)=C(C)C=2)=C1)N.Cl.O | [CAS DataBase Reference]
207738-08-7 |
| Hazard Information | Back Directory | [Chemical Properties]
Off-white fluffy powder | [Description]
TMB is an aromatic amine that undergoes oxidation by the higher oxidation states of heme peroxidases (compounds I and II) thereby serving as a reducing co-substrate. One electron oxidation of TMB results in a radical cation that forms a charge-transfer complex with the unoxidized compound. This charge transfer complex absorbs at 652 nm (ε = 39,000).1 The completely oxidized form (diimine) absorbs at 450 nm (ε = 59,000) and is formed by two sequential one-electron oxidations of TMB.1,2 Thus the stoichiometry of oxidation is 0.5 mole charge transfer complex (λmax = 652 nm) or 1 mole of diimine (λmax = 450 nm) formed (or TMB oxidized) per mole of hydroperoxide reduced by the peroxidase. | [Uses]
3,3',5,5'-Tetramethylbenzidine dihydrochloride hydrate is a chromophore substrate in ELISA assays. It is a noncarcinogenic substitute for benzidine. | [General Description]
3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate (TMB-d) is a sensitive chromogen substrate for peroxidase. It is non- carcinogenic, hence, it can be used as a safe substitute for benzidine. TMB-d is useful in quantifying hemoglobin and in staining myeloperoxidase-containing granules in granulocytes. | [storage]
Store at -20°C | [References]
[1] PANAKKEZHUM D. THOMAS Mark J P. A modified tetramethylbenzidine method for measuring lipid hydroperoxides[J]. Analytical biochemistry, 1990, 188 1: Pages 228-232. DOI: 10.1016/0003-2697(90)90557-p
[2] LEAH A. MARQUEZ H. B D. Mechanism of the Oxidation of 3,5,3‘,5‘-Tetramethylbenzidine by Myeloperoxidase Determined by Transient- and Steady-State Kinetics?[J]. Biochemistry Biochemistry, 1997, 36 31: 9349-9355. DOI: 10.1021/bi970595j
[3] P. JOSEPHY R M T Eling. The horseradish peroxidase-catalyzed oxidation of 3,5,3’,5’-tetramethylbenzidine. Free radical and charge-transfer complex intermediates.[J]. The Journal of Biological Chemistry, 1982, 31 1: 3669-3675. DOI: 10.1016/s0021-9258(18)34832-4
[4] S. MADERSBACHER P. B. Double wavelength measurement of 3,3′,5,5′-tetramethylbenzidine (TMB) provides a three-fold enhancement of the ELISA measuring range[J]. Journal of immunological methods, 1991, 138 1: Pages 121-124. DOI: 10.1016/0022-1759(91)90071-m
[5] ARMANDO REYNA-BELLO Adriana M S Mariana C Eleizalde. Assessment of chromogen suitability in ELISA for the detection of anaplasmosis and trypanosomosis.[J]. Journal of immunoassay & immunochemistry, 2007, 28 1: 1-11. DOI: 10.1080/15321810601025432 |
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