| Identification | Back Directory | [Name]
H-Gly-Gly-AMC | [CAS]
208645-74-3 | [Synonyms]
GG-AMC
Gly-Gly-AMC
Gly-Gly-MCA
H-Gly-Gly-AMC
Glycyl-N-(4-methyl-2-oxo-2H-1-benzopyran-7-yl)glycinamide
Glycinamide, glycyl-N-(4-methyl-2-oxo-2H-1-benzopyran-7-yl)- | [Molecular Formula]
C14H15N3O4 | [MOL File]
208645-74-3.mol | [Molecular Weight]
289.29 |
| Chemical Properties | Back Directory | [Boiling point ]
650.6±55.0 °C(Predicted) | [density ]
1.372±0.06 g/cm3(Predicted) | [solubility ]
DMF: 1 mg/ml; DMSO: 20 mg/ml; Ethanol: 1 mg/ml; PBS (pH 7.2): 0.8 mg/ml | [form ]
A crystalline solid | [pka]
12.63±0.20(Predicted) |
| Hazard Information | Back Directory | [Description]
Gly-Gly-AMC is a fluorogenic peptide substrate. It has been used to assess the activity of bacterial proteases from P. aeruginosa and S. aureus.1 Activity can be quantified by fluorescent detection of free AMC (also known as 7-amino-4-methylcoumarin), which is excited at 340-360 nm and emits at 440-460 nm.
| [Uses]
Gly-Gly-AMC is a fluorogenic peptide substrate. Gly-Gly-AMC can be used to assess bacterial protease activity of P. aeruginosa and S. aureus[1][2]. | [References]
1. Wildeboer, D., Jeganathan, F., Price, R.G., et al. Characterization of bacterial proteases with a panel of fluorescent peptide substrates Anal. Biochem. 384(2),321-328(2009). |
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