| Identification | Back Directory | [Name]
AC-IETD-AFC | [CAS]
211990-57-7 | [Synonyms]
AC-IETD-AFC
AC-ILE-GLU-THR-ASP-AFC
Caspase 8 Substrate 1f
CGUOWXRXLQXOKO-FCQBACSVSA-N
M.W. 729.66 C31H38F3N5O12
CASPASE-8 FLUOROGENIC SUBSTRATE
CASPASE 8 SUBSTRATE 1F, FLUOROGENIC
GRANZYME B SUBSTRATE I, COLORIMETRIC
AC-ILE-GLU-THR-ASP-7-AMINO-4-TRIFLUORO-METHYLCOUMARIN
N-ACETYL-ILE-GLU-THR-ASP-(7-AMINO-4-TRIFLUOROMETHYLCOUMARIN)
N-ACETYL-ILE-GLU-THR-ASP-7-AMINO-4-TRIFLUOROMETHYLCOUMARIN AFC
L-α-Asparagine, N-acetyl-L-isoleucyl-L-α-glutamyl-L-threonyl-N-[2-oxo-4-(trifluoromethyl)-2H-1-benzopyran-7-yl]-
N-Acetyl-L-isoleucyl-L-alpha-glutamyl-L-threonyl-N-[2-oxo-4-(trifluoromethyl)-2H-1-benzopyran-7-yl]-L-alpha-asparagine | [Molecular Formula]
C31H38F3N5O12 | [MDL Number]
MFCD02094703 | [MOL File]
211990-57-7.mol | [Molecular Weight]
729.65 |
| Chemical Properties | Back Directory | [Boiling point ]
1113.8±65.0 °C(Predicted) | [density ]
1.437±0.06 g/cm3(Predicted) | [storage temp. ]
-20°C | [solubility ]
Soluble in DMSO | [form ]
Lyophilized powder | [pka]
4.09±0.10(Predicted) | [color ]
White to off-white | [Appearance]
Solid Powder |
| Hazard Information | Back Directory | [Description]
Ac-IETD-AFC is a fluorogenic substrate for caspase-8 and caspase-3. Caspase activity can be quantified by fluorescent detection of free AFC (7-amino-4-trifluoromethylcoumarin), which is excited at 400 nm and emits at 505 nm. | [Uses]
Ac-IETD-AFC is a fluorogenic substrate for caspase-8/caspase-3 processing enzyme and granzyme B. It is also a substrate for caspase-10. The IETD sequence is based on caspase-3 proenzyme cleavage site. | [Uses]
N-Acetyl-Ile-Glu-Thr-Asp-7-amino-4-(trifluoromethyl)coumarin is a fluorogenic substrate for caspases -6, -8, and -10. The IETD sequence is based on the procaspase-3 cleavage site. Residues 172-175 of procaspase-3 are cleaved during apoptosis to produce the p12 subunit and a p20 peptide that is further cleaved to form the p17 subunit of mature caspase 3. | [Excitation / Emission Maximum]
400 nm/505 nm | [Sequene]
Ac-Ile-Glu-Thr-Asp-Unk |
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| Company Name: |
Energy Chemical
|
| Tel: |
021-58432009 400-005-6266 |
| Website: |
http://www.energy-chemical.com |
|