| Identification | Back Directory | [Name]
AC-IEPD-PNA | [CAS]
216757-29-8 | [Synonyms]
AC-IEPD-PNA
AC-ILE-GLU-PRO-ASP-PNA
AC-ILE-GLU-PRO-ASP-PARANITROANILIDE
GRANZYME B SUBSTRATE VIII, COLORIMETRIC
N-Acetyl-Ile-Glu-Pro-Asp-p-nitroanilide
N-ACETYL-ILE-GLU-PRO-ASP-P-NITROANILINE
N-ACETYL-ILE-GLU-PRO-ASP-P-NITROANILIDE;AC-IETD-PNA
L-α-Asparagine, N-acetyl-L-isoleucyl-L-α-glutamyl-L-prolyl-N-(4-nitrophenyl)- (9CI) | [Molecular Formula]
C28H38N6O11 | [MDL Number]
MFCD02259221 | [MOL File]
216757-29-8.mol | [Molecular Weight]
634.63 |
| Chemical Properties | Back Directory | [Boiling point ]
1096.9±65.0 °C(Predicted) | [density ]
1.386±0.06 g/cm3(Predicted) | [storage temp. ]
−20°C | [solubility ]
DMSO: soluble | [form ]
powder | [pka]
4.06±0.10(Predicted) | [color ]
White to off-white | [Sequence]
Ac-Ile-Glu-Pro-Asp-{pNA} | [InChIKey]
YXEIIOVAGGAMCZ-NYMCBPKFSA-N | [SMILES]
CC[C@H](C)[C@H](NC(C)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CC(O)=O)C(=O)Nc2ccc(cc2)[N+]([O-])=O |
| Hazard Information | Back Directory | [Uses]
N-Acetyl-Ile-Glu-Pro-Asp-p-nitroanilide (Ac-IEPD-pNA) is a granzyme B substrate that allows accurate measurement of granzyme B activity[1]. | [Biological Activity]
Primary Target
Colorimetric substrate for granzyme B''kcat/Km = 6.6 x 104 M-¹ sec-¹ as substrate for granzyme B | [References]
[1] Kristina Fritsch, et al. Suppression of granzyme B activity and caspase-3 activation in leukaemia cells constitutively expressing the protease inhibitor 9. Ann Hematol. 2013 Dec;92(12):1603-9. DOI:10.1007/s00277-013-1846-6 |
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