| Chemical Properties | Back Directory | [solubility ]
Soluble in methanol, DMSO, DMF | [Appearance]
Yellow solid | [ex/em]
490/513 nm | [ε(extinction coefficient)]
80,000 L⋅mol−1⋅cm−1 | [Φ(quantum yield)]
0.93 |
| Hazard Information | Back Directory | [Description]
FAM amine, 6-isomer is a fluorescein linker that undergoes enzymatic transamination. The addition of the amine group makes the compound more reactive with carboxylic acids, activated NHS ester and other carbonyl groups. | [Chemical Properties]
Appearance: Yellow solid
ex/em: 490/513 nm
Extinction coefficient (ε): 80,000 L??mol??1??cm??1
Quantum yield (Φ): 0.93 | [Uses]
FAM amine, 6-isomer is a fluorescein derivative with an amine group and contains an isomer of the fluorophore. Can be used to modify biomolecules through enzymatic transamination. Its fatty amine groups can also react with electrophiles such as activated esters. The amine can also be conjugated to carbonyl compounds (aldehydes and ketones) by reductive amination. | [References]
[1] Hung S C, Ju J, Mathies R A, et al. Cyanine dyes with high absorption cross section as donor chromophores in energy transfer primers1[J]. Analytical biochemistry, 1996, 243(1): 15-27. | [Abs/Em Maxima]
490/513 nm | [Fluoresene quantum yield]
0.93 | [Extinction Coefficient]
80000 | [CF260]
0.2 | [CF280]
0.17 |
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