| Hazard Information | Back Directory | [Description]
Cy3 Azide can be reacted with terminal alkynes via a copper-catalyzed click reaction (CuAAC). It also reacts with strained cyclooctyne via a copper-free “click chemistry” reaction to form a stable triazole and does not require Cu-catalyst or elevated temperatures. This red fluorescent probe is water-soluble, and its fluorescence is pH-insensitive from pH 4 to pH 10. Its excitation peak is ideally suited for the 532 nm or 555 nm laser lines and its absorption and emission spectra are almost identical to those of Alexa Fluor® 555, CF® 555 Dye or any other Cyanine3 based fluorescent dyes. | [Uses]
Azide modified cyanine dye. Can be used to detect or label alkyne- or cyclooctyne-containing molecules or biomolecules by fluorescence spectroscopy following azide-alkyne cycloaddition.
Solubility -DMSO; water; DMF; MeOH
Abs/Em - 553/566 nm
Extinction Coefficient - 151; 000 cm-1M-1 | [reaction suitability]
reaction type: click chemistry | [Abs/Em Maxima]
553/569 nm | [Extinction Coefficient]
150,000 | [Microscopy Laser Line]
532 or 555 nm | [Flow Cytometry Laser Line]
532, 555 or 568 nm | [Spectrally Similar Dyes]
Alexa Fluor® 555, Atto™ 555, CF® 555 Dye, DyLight®549 |
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