| Identification | Back Directory | [Name]
1H-Benz[e]indolium, 2-[5-(1,3-dihydro-1,1,3-trimethyl-6,8-disulfo-2H-benz[e]indol-2-ylidene)-1,3-pentadien-1-yl]-3-[6-[(2,5-dioxo-1-pyrrolidinyl)oxy]-6-oxohexyl]-1,1-dimethyl-6,8-disulfo-, inner salt, potassium salt (1:3) | [CAS]
2419286-92-1 | [Synonyms]
Sulfo-Cyanine5.5 SE
1H-Benz[e]indolium, 2-[5-(1,3-dihydro-1,1,3-trimethyl-6,8-disulfo-2H-benz[e]indol-2-ylidene)-1,3-pentadien-1-yl]-3-[6-[(2,5-dioxo-1-pyrrolidinyl)oxy]-6-oxohexyl]-1,1-dimethyl-6,8-disulfo-, inner salt, potassium salt (1:3) | [Molecular Formula]
C44H46KN3O16S4 | [MOL File]
2419286-92-1.mol | [Molecular Weight]
1040.2 |
| Chemical Properties | Back Directory | [storage temp. ]
Store at -20°C, away from moisture | [solubility ]
易溶于DMSO，DMF等有机溶剂，极易溶于水 | [form ]
Solid | [color ]
Blue to dark blue | [Appearance]
Dark blue solid | [ex/em]
678/706 nm (PBS buffer) | [ε(extinction coefficient)]
211000 L⋅mol−1⋅cm−1 | [Φ(quantum yield)]
0.21 |
| Hazard Information | Back Directory | [Description]
sulfo-Cyanine5.5 NHS ester is an amine-reactive activated ester of sulfonated far-red Cyanine5.5 fluorophore, an analog of Cy5.5®. This is a reagent of choice for the labeling of antibodies, sensitive proteins, and others which require reactions in a purely aqueous environment or without any significant additions of organic co-solvents.
The dye is well-suitable for non-invasive in vivo NIR imaging and applications requiring low fluorescent background. | [Chemical Properties]
Appearance: Dark blue solid
ex/em: 678/706 nm (PBS buffer)
Extinction coefficient (ε): 211000 L??mol??1??cm??1
Quantum yield (Φ): 0.21 | [Uses]
Sulfo-CY-5.5 NHS ester (Sulfo-Cyanine5.5 NHS ester) tripotassium is an amine-reactive ester of sulfonated far-red Cyanine5.5 fluorophore, which can be used to label antibodies, proteins, etc., as well as for in vivo NIR imaging[1]. | [storage]
Store at -20°C, away from moisture | [References]
[1] Xiaoyu Xu, et al. Microfluidic-assisted biomineralization of CRISPR/Cas9 in near-infrared responsive metal-organic frameworks for programmable gene-editing. Nanoscale. 2022 Nov 3;14(42):15832-15844. DOI:10.1039/d2nr04095f | [Abs/Em Maxima]
675/694 nm | [Fluoresene quantum yield]
0.21 | [Extinction Coefficient]
211000 | [CF260]
0.09 | [CF280]
0.11 |
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