| Identification | Back Directory | [Name]
HHHHHH | [CAS]
64134-30-1 | [Synonyms]
HHHHHH
HIS TAG
6×
HEXA HIS
H2N-HHHHHH-OH
6 HIS PEPTIDE
Hexa-Histidine
HIS-HIS-HIS-HIS-HIS-HIS
H-HIS-HIS-HIS-HIS-HIS-HIS-OH
Hexa-His,His-His-His-His-His-His,HHHHHH
L-Histidyl-l-histidyl-l-histidyl-l-histidyl-l-histidyl-l-histidine
L-Histidine, L-histidyl-L-histidyl-L-histidyl-L-histidyl-L-histidyl- | [Molecular Formula]
C36H44N18O7 | [MDL Number]
MFCD02262140 | [MOL File]
64134-30-1.mol | [Molecular Weight]
840.85 |
| Hazard Information | Back Directory | [Uses]
Hexa-His (6X His Tag) is a commonly used affinity tag made up of six histidine residues. HEXA-HIS can bind to affinity chromatography media containing transition metal ions like nickel (Ni2+) or cobalt (Co2+), making it useful for protein purification[1][2]. | [References]
[1] Watly J, et al. Insight into the coordination and the binding sites of Cu(2+) by the histidyl-6-tag using experimental and computational tools. Inorg Chem. 2014 Jul 7;53(13):6675-83. DOI:10.1021/ic500387u
[2] Ana Paula de Mattos Arêas, et al. Synthesis of cholera toxin B subunit gene: cloning and expression of a functional 6XHis-tagged protein in Escherichia coli.Protein Expr Purif.2002 Aug;25(3):481-7. DOI:10.1016/s1046-5928(02)00026-8 |
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