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654651-21-5

654651-21-5 Structure

654651-21-5 Structure
IdentificationBack Directory
[Name]

BDP 581/591 NHS ester
[CAS]

654651-21-5
[Synonyms]

BDP 581/591 NHS ester
[Molecular Formula]

C26H22BF2N3O4
[MDL Number]

MFCD31580123
[MOL File]

654651-21-5.mol
[Molecular Weight]

489.29
Chemical PropertiesBack Directory
[solubility ]

Soluble in DMF, DMSO, DCM
[form ]

Solid
[color ]

Brown to black
[Appearance]

dark colored solid
Hazard InformationBack Directory
[Description]

BDP 581/591 NHS ester is a BDP dye linker that is used for fluorescence polarization assays. The diene portion of the compound makes it reactive with oxygen species. In addition, the introduction of the NHS ester group makes the compound more reactive with primary amines.
[Uses]

BDP 581/591 NHS ester is a a borondipyrromethene dye (Ex=585 nm, Em=594 nm) that has relatively long fluorescence lifetime and two photon excitation cross section. BDP 581/591 NHS ester can be used for fluorescence polarization analysis and also reacts with reactive oxygen species (ROS) and alter fluorescence. BDP 581/591 NHS ester is also an NHS ester derivative that can be used to bind primary and secondary amine groups of proteins, peptides and other molecules.
[Abs/Em Maxima]

585/594 nm
[Fluoresene quantum yield]

0.83
[Extinction Coefficient]

104000
[CF260]

0.06
[CF280]

0.04
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