| Identification | Back Directory | [Name]
N/A | [CAS]
691364-49-5 | [Synonyms]
Recaldent
Casein calcium oeotide
Casein peptide phosphate
CaseinPhosphopeptides/CCP
Casein Phosphopeptide (CPP)
Casein calcium oeotide(CCP)
Casein phosphopeptide USP/EP/BP | [EINECS(EC#)]
1592732-453-0 |
| Hazard Information | Back Directory | [Chemical Properties]
CPP is obtained by hydrolyzing cow's milk with trypsin to release CPP, which is then debittered using ion exchange resins and purified by membrane separation. α-CPP originates from α-casein, while β-CPP originates from β-casein. Depending on the purification method, a variety of products can be produced. The manufacturing process for Meiji Seika Co., Ltd. in Japan is as follows: Zheng Tiesong of Zhejiang University uses the isoelectric precipitation method to obtain casein from whey. Using calcium retention capacity as an indicator in a positive efficacy test, the optimal conditions were determined: casein was prepared into a 15% solution, adjusted to a pH of approximately 8.3 with 2 mol/L NaOH, and hydrolyzed with trypsin at a substrate-to-enzyme ratio of 100:1. The pH of the solution was maintained between 7.7 and 8.5 throughout the hydrolysis process, at a temperature of 40°C. After 80 minutes of hydrolysis, 1 mol/L HCl was added to bring the pH of the solution to 4.8. The precipitate was removed by centrifugation, and 10% CaCl2 solution was added to the supernatant to a final concentration of 100 mmol/L. The solution was washed with ether, evaporated, and dried to obtain the finished product. The yield was 9.78%, and the calcium retention capacity (P:N) was 0.355. | [Uses]
Casein phosphopeptide is a biochemical reagent that can be used as a biological material or organic compound for life science related research. |
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