| Identification | Back Directory | [Name]
PERTUSSIS TOXIN | [CAS]
70323-44-3 | [Synonyms]
iap
PTX
ALPI
IAG2
MAGT1
PSEC0084
HOLOTOXIN
pertussigen
UNQ628/PRO1244
pertussistoxins
PERTUSSIS TOXIN
toxins,pertussis
ISLET ACTIVATING PROTEIN
BORDETELLA PERTUSSIS TOXIN
histamine-sensitizingfactor
lymphocytosis-promotingfactor
Implantation-associated protein
Magnesium transporter protein 1
POLYETHYLENE GLYCOL AV. MOL. WT. 200
PERTUSSIS TOXIN, BORDETELLA PERTUSSIS
Bordetella pertussis toxin
DISCONTINUED
Pertussis toxin from Bordetella pertussis
pertussis toxin from bordetella per-tussis
ANTI-MAGT1 (N-TERM) antibody produced in rabbit
PERTUSSIS TOXIN FROM BORDETELLA PER-TUSS IS, 50 UG TOXIN
ANTI-ALKALINE PHOSPHATASE (ALPI) (CENTER antibody produced in rabbit
Histamine-sensitizing factor, IAP, Islet Activating Protein, Pertussigen
Pertussis toxin from Bordetella pertussis,Histamine-sensitizing factor, IAP, Islet Activating Protein, Pertussigen
Protein-based AB5-type exotoxin with five dissimilar subunits: S-1 (M.W. 28 kDa), S-2 (M.W. 23 kDa), S-3 (M.W. 22 kDa), S-4 (M.W. 11.7 kDa) and S-5 (M.W. 9.3 kDa), in a molar ratio of 1:1:1:2:1 | [Molecular Formula]
NULL | [MDL Number]
MFCD00131841 |
| Chemical Properties | Back Directory | [storage temp. ]
2-8°C | [solubility ]
Water: soluble | [form ]
lyophilized powder
| [color ]
White to off-white | [InChI]
1S/C26H52O2/c1-3-4-5-6-7-8-9-10-11-12-13-14-15-16-17-18-19-20-21-22-23-24-25-26(27)28-2/h3-25H2,1-2H3 | [InChIKey]
WOPKHAQDUMDJIY-UHFFFAOYSA-N | [SMILES]
O(C)C(=O)CCCCCCCCCCCCCCCCCCCCCCCC |
| Hazard Information | Back Directory | [Uses]
The toxin is released from B. pertussis in an inactive form. When the pertussis toxin B oligomer binds to the cell membrane, the S1 subunit of its A protomer becomes activated, perhaps through the action of glutathione and ATP. A protocol for activating pertussis toxin in vitro is given by Kaslow, et al. | [General Description]
Product is not activated. If activation is required, see Kaslow, et al., for suggested conditions. | [Biochem/physiol Actions]
Pertussis toxin catalyzes the ADP-ribosylation of the α subunits of the heterotrimeric guanine nucleotide regulatory proteins Gi, Go, and Gt. This prevents the G protein heterotrimers from interacting with receptors, thus blocking their coupling and activation. Since the Gα subunits remain in their GDP-bound, inactive state, they are unable to inactivate adenylyl cyclase or open K+ channels. | [storage]
Store at +4°C | [Purification Methods]
Purify the toxin by stepwise elution from 3 columns comprising Blue Sepharose, Phenyl Sepharose and hydroxylapatite, and the purity is checked by SDS-PAGE. [Svoboda et al. Anal Biochem 159 402 1986, Biochemistry 21 5516 1982, Biochem J 83 295 1978.] |
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