| Identification | Back Directory | [Name]
N-METHOXYSUCCINYL-ALA-ALA-PRO-VAL P-NITROANILIDE | [CAS]
70967-90-7 | [Synonyms]
M4765
MeOSuc-AAPV-pNA
MEOSUC-ALA-ALA-PRO-VAL-PNA
LEUKOCYTE ESTERASE SUBSTRATE
ELASTASE SUBSTRATE I, COLORIMETRIC
MeOSuc-AAPV-pNA, Chromogenic Substrate
MEO-SUC-ALA-ALA-PRO-VAL-PARANITROANILIDE
METHOXYSUCCINYL-ALA-ALA-PRO-VAL-P-NITROANILIDE
N-METHOXYSUCCINYL-ALA-ALA-PRO-VAL P-NITROANILIDE
N-(Methoxysuccinyl)-Ala-Ala-Pro-Val 4-nitroanilide
N(methoxysuccinyl)-L-alanyl-L-alanyl-L-prolyl-L-valine NA
N-methoxysuccinyl-alanyl-alanyl-prolyl-valine-4-nitroanilide
N-METHOXYSUCCINYL-ALA-ALA-PRO-VAL P-NITROANILIDE, BIOCHEMIKA
METHOXYSUCCINYL-ALA-ALA-PRO-VAL-P-NITROANILIDE, METHANOL ADDUCT
Elastase Substrate I, Colorimetric - CAS 70967-90-7 - Calbiochem
N-(METHOXYSUCCINYL)-L-ALANYL-L-ALANYL-L-PROLYL-L-VALINE 4-NITROANILIDE
Methoxysuccinyl-Ala-Ala-Pro-Val-p-nitroanilide - CAS 70967-90-7 - Calbiochem
L-Valinamide, N-(4-methoxy-1,4-dioxobutyl)-L-alanyl-L-alanyl-L-prolyl-N-(4-nitrophenyl)-
N-(Methoxysuccinyl)-Ala-Ala-Pro-Val 4-nitroanilide, N-(Methoxysuccinyl)-L-alanyl-L-alanyl-L-prolyl-L-valine 4-nitroanilide
methyl 4-[[(2S)-1-[[(2S)-1-[(2S)-2-[[(2S)-3-methyl-1-(4-nitroanilino)-1-oxobutan-2-yl]carbamoyl]pyrrolidin-1-yl]-1-oxopropan-2-yl]amino]-1-oxopropan-2-yl]amino]-4-oxobutanoate | [EINECS(EC#)]
232-670-7 | [Molecular Formula]
C27H38N6O9 | [MDL Number]
MFCD00077137 | [MOL File]
70967-90-7.mol | [Molecular Weight]
590.63 |
| Chemical Properties | Back Directory | [Melting point ]
203-205°C | [Boiling point ]
958.4±65.0 °C(Predicted) | [density ]
1.297±0.06 g/cm3(Predicted) | [storage temp. ]
-20°C | [solubility ]
DMF: 25 mg/mL, clear, light yellow | [form ]
Off-white solid | [pka]
13.29±0.20(Predicted) | [color ]
White to Off-White | [Appearance]
White Solid | [Water Solubility ]
water: 1mg/mL | [BRN ]
6266359 | [InChIKey]
YEGSVRNITOBIRO-OSAZLGQLSA-N | [SMILES]
C(N)(=O)[C@H](C(C)C)N(C(=O)[C@@H]1CCCN1C(=O)[C@H](C)NC(=O)[C@H](C)NC(=O)CCC(OC)=O)C1=CC=C([N+]([O-])=O)C=C1 |
| Hazard Information | Back Directory | [Description]
Proteinase 3 (PR3, myeloblastin) is a polymorphonuclear leukocyte serine proteinase that degrades matrix proteins including fibronectin, laminin, vitronectin, and collagen type IV to generate antimicrobial peptides. Neutrophil elastase is a serine proteinase that is secreted by neutrophils during inflammation to destroy pathogens. Evaluating these enzymes is helpful to understanding inflammatory autoimmune processes. MeOSuc-AAPV-pNA is a highly sensitive peptide substrate that is hydrolyzed by both human and mouse neutrophil elastase and PR3, but not cathepsin G or chymotrypsin. Enzyme activity can be quantified by colorimetric detection of free p-nitroanilide at 405 nm. | [Uses]
N-Methoxysuccinyl-Ala-Ala-Pro-Val p-Nitroanilide is a highly sensitive peptide substrate that is hydrolyzed by both human and mouse neutrophil elastase and PR3 but not cathespin. | [storage]
Store at -20°C | [References]
[1] OLAF WIESNER . Differences between human proteinase 3 and neutrophil elastase and their murine homologues are relevant for murine model experiments[J]. FEBS Letters, 2005, 579 24: Pages 5305-5312. DOI: 10.1016/j.febslet.2005.08.056
[2] B. KORKMAZ. Discriminating between the Activities of Human Neutrophil Elastase and Proteinase 3 Using Serpin-derived Fluorogenic Substrates*[J]. The Journal of Biological Chemistry, 2002, 7 1: 39074-39081. DOI: 10.1074/jbc.m202918200 | [Sequene]
MeOSuc-Ala-Ala-Pro-Val-pNA |
|
|