| Identification | Back Directory | [Name]
PENTAFLUOROBENZENESULFONYL FLUORESCEIN | [CAS]
728912-45-6 | [Synonyms]
UIBLLPHZQUTIBL-UHFFFAOYSA-N
PENTAFLUOROBENZENESULFONYL FLUORESCEIN
6'-HYDROXY-3-OXASPIRO[ISOBENZOFURAN-1(3H),9'-[9H]XANTHEN]-3'-YL ESTER, PENTAFLUOROBENZENESULFONIC ACID
Benzenesulfonic acid, 2,3,4,5,6-pentafluoro-, 6'-hydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3'-yl ester | [Molecular Formula]
C26H11F5O7S | [MDL Number]
MFCD08062167 | [MOL File]
728912-45-6.mol | [Molecular Weight]
562.42 |
| Chemical Properties | Back Directory | [storage temp. ]
Store at -20°C | [solubility ]
DMF: 30 mg/ml; DMSO: 30 mg/ml; DMSO:PBS (pH 7.2)(1:1): .25 mg/ml; Ethanol: 20 mg/ml | [form ]
A crystalline solid | [color ]
Light yellow to yellow | [Appearance]
Solid Powder |
| Hazard Information | Back Directory | [Description]
Reactive oxygen species (ROS) play important roles in the initiation and progression of many disease processes. Most fluorescent probes for ROS detection, such as 2,7-dichlorofluorescein (DCFH), function by an oxidative mechanism and are useful for total oxidant detection but are not selective for hydrogen peroxide specifically.1 Pentafluorobenzenesulfonyl fluorescein is a fluorescent probe that functions by a nonoxidative mechanism. It fluoresces upon perhydrolysis of the sulfonyl linkage, and is selective for hydrogen peroxide over hydroxyl radical, tert-butyl hydroperoxide, superoxide anion, singlet oxygen, and nitrates. Pentafluorobenzenesulfonyl fluorescein can be used as a probe for hydrogen peroxide in whole cell systems.1 | [Uses]
Pentafluorobenzenesulfonyl fluorescein is a H2O2-selective sensor that can be used to detect H2O2 levels in cells. Pentafluorobenzenesulfonyl fluorescein is normally non-fluorescent but fluoresces upon perhydrolysis of the sulfonyl linkage by H2O2[1]. | [References]
1. Maeda, H., Fukuyasu, Y., Yoshida, S., et al. Fluorescent probes for hydrogen peroxide based on a non-oxidative mechanism Angew. Chem. Int. Ed. Engl. 43(18),2389-2391(2004). | [Excitation / Emission Maximum]
465-505 nm/505-555 nm |
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