| Identification | Back Directory | [Name]
NUCLEAR YELLOW | [CAS]
74681-68-8 | [Synonyms]
approx.80%
Hoechst
s769121
Nuclear yellow, BR
NUCLEAR YELLOW APPROX. 80%
Hoechst S769121 Trihydrochloride
Nuclear Yellow [Hoechst S769121] *CAS 74681-68-8*
4-[5-(4-methyl-1-piperazinyl)[2,50-bi-1hbenzimidazol]-
20-yl]-benzenesulfonamide
Benzenesulfonamide, 4-[5-(4-methyl-1-
piperazinyl)[2,50-bi-1h-benzimidazol]-20-yl]-, Trihydrochloride | [Molecular Formula]
C25H25N7O2S.3ClH | [MDL Number]
MFCD00037016 | [MOL File]
74681-68-8.mol | [Molecular Weight]
596.968 |
| Chemical Properties | Back Directory | [Melting point ]
>300℃ | [density ]
1.3423 (rough estimate) | [refractive index ]
1.6000 (estimate) | [storage temp. ]
Inert atmosphere,Store in freezer, under -20°C | [solubility ]
Soluble in dimethyl sulfoxide, water | [form ]
powder | [color ]
Yellow to green | [λmax]
355 nm | [Biological Applications]
Detecting nucleic acid,proteins,carbohydrates,lipids; carrying out polymerase chain reaction (PCR); drug-targeting agent; nerve mapping |
| Hazard Information | Back Directory | [Uses]
Hoechst S 769121 is a marker dye in Hoechst series. Hoechst is A live nuclear marker dye. Hoechst binds to the grooves in the DNA double strand, which tends to be A/ T-rich DNA strand. Although it binds to all nucleic acids, the A/ T-rich double strand DNA significantly enhances fluorescence intensity Therefore,Hoechst dye can be used for living cell labeling. The fluorescence intensity of Hoechst dye increases with the increase of pH of solution[1]. | [References]
[1] Latt SA, Stetten G, Juergens LA, Recent developments in the detection of deoxyribonucleic acid synthesis by 33258 Hoechst fluorescence. The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 23 (7): 493-505. DOI:10.1177/23.7.1095650
[2] a b c "Hoechst Stains". Invitrogren (Molecular Probes).
[3] Portugal J, Waring MJ. Assignment of DNA binding sites for 4',6-diamidine-2-phenylindole and bisbenzimide (Hoechst 33258). A comparative footprinting study. Biochimica et Biophysica Acta 949 DOI:10.1016/0167-4781(88)90079-6 |
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Musechem
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+1-800-259-7612 |
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www.musechem.com |
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