| Identification | Back Directory | [Name]
N-P-TOSYL-GLY-PRO-ARG P-NITROANILIDE ACETATE SALT | [CAS]
86890-95-1 | [Synonyms]
Einecs 289-285-2
Thrombin Substrate I
N-(p-Tosyl)-GPR-pNA (acetate)
N-P-TOSYL-GLY-PRO-ARG P-NITROANILIDE
N-Tosyl-Gly-Pro-Arg 4-nitroanilide acetate
N-P-TOSYL-GLY-PRO-ARG P-NITROANILIDE*ACE TATE
Tosyl-Gly-Pro-Arg-(4-nitrophenyl)NH2·acetic acid
N-P-TOSYL-GLY-PRO-ARG P-NITROANILIDE ACETATE SALT
N (p Tosyl) GPR pNA acetate,N(pTosyl)GPRpNA acetate
N-tosylglycyl-L-prolyl-L-arginine 4-nitroanilide acetat
N-TOSYLGLYCYL-L-PROLYL-L-ARGININE 4-NITROANILIDE ACETATE
n-tosylglycyl-l-prolyl-l-arginine 4-nitroanilide acetate salt
N-tosylglycyl-L-prolyl-N-(p-nitrophenyl)-L-argininamide monoacetate
L-Argininamide, N-((4-methylphenyl)sulfonyl)glycyl-L-propyl-N-(4-nitrophenyl)-, monoacetate | [EINECS(EC#)]
289-285-2 | [Molecular Formula]
C28H38N8O9S | [MDL Number]
MFCD00058169 | [MOL File]
86890-95-1.mol | [Molecular Weight]
662.71 |
| Hazard Information | Back Directory | [Description]
N-(p-Tosyl)-GPR-pNA is a colorimetric substrate for thrombin. Thrombin preferentially binds to and cleaves the Gly-Pro-Arg (GPR) peptide sequence to release p-nitroanilide (pNA), which can be quantified by colorimetric detection at 405 nm as a measure of thrombin activity. | [Uses]
N-(p-Tosyl)-GPR-pNA acetate (Chromozym-TH) is a chromogenic substrate targeting the synthetic peptides Hirunorm IV and Hirunorm V and can be used to detect the dissociation constants (KI) of both peptides. Hirunorm IV and Hirunorm V are reversible inhibitors of amidolytic thrombin activity. By varying the peptide concentration at a fixed concentration of the chromogenic substrate N-(p-Tosyl)-GPR-pNA acetate, the dissociation constants determined were 0.134 nM (Hirunorm IV) and 0.245 nM (Hirunorm V)[1]. | [storage]
Store at -20°C | [References]
[1] Cappiello M, et al. Kinetics of human thrombin inhibition by two novel peptide inhibitors (Hirunorm IV and Hirunorm V). Biochem Pharmacol. 1996 Oct 25;52(8):1141-6. DOI:10.1016/0006-2952(96)00388-7 |
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