| Identification | Back Directory | [Name]
EC 2.7.3.2 | [CAS]
9001-15-4 | [Synonyms]
CK
CPK
PCPK
RCPK
Mib-CK
S-MtCK
EC 2.7.3.2
EC: 2.7.3.2
CREATINE KINASE
Creatine kinase-2
CK diagnostic kit.
CREATINE PHOSPHOKINASE
CREATINE KINASE TYPE I
CREATINE KINASE TYPE IV
CREATINE KINASE TYPE III
sarcomeric mitochondrial
CreatineKinaseExRabbitMuscle
Adenylate kinase isoenzyme 1
ATP-AMP transphosphorylase 1
CREATINE PHOSPHOKINASE TYPE I
PHOSPHOCREATINE PHOSPHOKINASE
CREATINE PHOSPHOKINASE, RABBIT
CREATINE PHOSPHOKINASE TYPE IV
creatine kinase diagnostic kit
CREATINE PHOSPHOKINASE TYPE III
CREATINE PHOSPHOKINASE, PORCINE
CPK:frombovineheartorrabbitmuscle
CREATINE KINASE, FROM BOVINE HEART
ATP: CREATINE N-PHOSPHOTRANSFERASE
Kinase (phosphorylating), creatine
PHOSPHOCREATINE PHOSPHOKINASE TYPE I
creatine phosphokinase(rabbit music)
reatine phosphokinase (rabbit music)
creatine phosphokinase, rabbit muscle
PHOSPHOCREATINE PHOSPHOKINASE TYPE IV
PHOSPHOCREATINE PHOSPHOKINASE TYPE III
creatine phosphokinase from bovine heart
creatine phosphokinase from rabbit brain
Basic-type mitochondrial creatine kinase
CPK/Creatine phosphokinase(rabbit Muscle)
creatine phosphokinase from rabbit muscle
ATP: CREATINE N-PHOSPHOTRANSFERASE TYPE I
ATP: CREATINE N-PHOSPHOTRANSFERASE TYPE IV
ATP: CREATINE N-PHOSPHOTRANSFERASE TYPE III
CREATINE PHOSPHOKINASE, RABBIT SKELETAL MUSCLE
Creatine kinase from rabbit muscle ca. 240 U/mg
CREATINE PHOSPHOKINASE TYPE IV FROMRABBI T BRAIN
CREATINE PHOSPHOKINASE TYPE I FROMRABBIT MUSCLE
CREATINE PHOSPHOKINASE TYPE III FROMBOVI NE HEART
CREATINE PHOSPHOKINASE FROM RABBIT MUSCL E, ~20 U/MG
CREATINE PHOSPHOKINASE FROM RABBIT MUSCL E, ~14 U/MG
CREATINE KIN. FR. RABBIT MUSC.CA.240 U/MG LYOPH. SALT-FREE
Creatine kinase (CK-BB iso-enzyme) (catalytic concentration)
Creatine phosphokinase, from rabbit muscle, typeI, >=150 units/mg
Creatine kinase-2 (CK-MB isoenzyme) (catalytic concentration)
78861, Creatine kinase (CK-BB iso-enzyme ) (catalytic concentration)
ATP: Creatine N-Phosphotransferase, CPK, Creatine Kinase, Phosphocreatine phosphokinase
Creatine Phosphokinase from rabbit brain,ATP: Creatine N-Phosphotransferase, CPK, Creatine Kinase, Phosphocreatine phosphokinase
Creatine Phosphokinase from bovine heart,ATP: Creatine N-Phosphotransferase, CPK, Creatine Kinase, Phosphocreatine phosphokinase
Creatine Phosphokinase from rabbit muscle,ATP: Creatine N-Phosphotransferase, CPK, Creatine Kinase, Phosphocreatine phosphokinase | [EINECS(EC#)]
232-585-5 | [Molecular Formula]
NULL | [MDL Number]
MFCD00130876 |
| Chemical Properties | Back Directory | [storage temp. ]
−20°C
| [solubility ]
0.25 M glycyl-glycine, pH 7.4: soluble5.0mg/mL, clear, colorless to slightly yellow | [form ]
salt-free, lyophilized powder
| [color ]
white
| [biological source]
rabbit skeletal muscle | [Water Solubility ]
aqueous buffer: soluble
water: soluble | [Specific Activity]
≥30units/mg protein | [CAS DataBase Reference]
9001-15-4 | [EPA Substance Registry System]
Kinase (phosphorylating), creatine (9001-15-4) |
| Hazard Information | Back Directory | [Chemical Properties]
This is an off-white, salt-free lyophilized powder, soluble in water, with an optimal pH of 6.0-7.0 (forward reaction) and 9.0 (reverse reaction). Stability: Inactivated at a pH below 5, and thawing and freezing also inactivate the enzyme. At -20°C and a pH of 6.6, it is stable in 0.1 molar mass/L imidazole buffer for four weeks. It is most stable in a neutral 60% ethanol solution. Adding 0.1% albumin or β-mercaptoethanol to dilute solutions can increase stability. The freeze-dried product can be stored for 12 months at -20°C, but loses 15% activity after six months at 4°C. Inhibitors include para-chloromercuric benzoate, chelating agents, iodoacetic acid, tripolyphosphate, adenosine, nitrite, sulfate, bromide, sulfite, chloride, fluoride, thyroxine, 3,5-dinitro-o-cresol, coenzyme I (NAD), L-triiodoformyl amino acid (T3), malonate, iodine (I-), iodoacetic acid, orthophosphoric acid, pyrophosphate, and tripolyphosphate. Activators include Mg2+, Mn2+, 2-mercaptoethanol, dithiothreitol, glutathione, and the thiol N-acetylcysteine. The absorbance coefficient of a 1% aqueous solution at 280 nm is 8.76. The enzyme reaction is: creatine + adenosine triphosphate = adenosine diphosphate + creatine phosphate. | [Uses]
The enzyme from Sigma has been used in the measurement of phosphocreatine in hippocampal neurons isolated from rat brains. | [Biochem/physiol Actions]
Creatine kinase plays a key role in the energy metabolism of cells with intermittently high and fluctuating energy requirements. Examples of such cells include cardiac or skeletal muscle cells and neural tissues of brain and retina. The enzyme catalyzes the reversible transfer of the phosphoryl group from phosphorylcreatine to ADP, in order to generate ATP. The molecular mass of the protein is found to be approximately 80,000 Da. It is made up of 2 subunits, each having a molecular weight of 40,000 ± 2000. The lighter subunit is present in larger amounts. |
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Maya High Purity Chemicals
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