| Identification | Back Directory | [Name]
PROTHROMBIN | [CAS]
9001-26-7 | [Synonyms]
FACTOR II
PROTHROMBIN
HUMAN PROTHROMBIN
PROTHROMBIN, HUMAN
PROTHROMBIN (BOVINE)
PROTHROMBIN, HUMAN PLASMA
III-2isoelec.pointatpH4.2
Human Prothrombin Complex
factor ii from human plasma
Blood-coagulation factor II
COAGULATION FACTOR II, HUMAN
PROTHROMBIN FROM HUMAN PLASMA
ProthrombinfrombovineplasmafractionIII-2
factor ii (prothrombin) from human*plasma | [EINECS(EC#)]
232-592-3 | [MDL Number]
MFCD00166309 |
| Chemical Properties | Back Directory | [Definition]
The precursor of the enzyme
thrombin, a proteinaceous component of blood
plasma that is converted into thrombin by the blood-
clotting mechanism when activated at the site of an
open wound.
| [storage temp. ]
−20°C
| [solubility ]
water: soluble | [form ]
lyophilized powder
| [Water Solubility ]
water: soluble |
| Hazard Information | Back Directory | [Uses]
Human Prothrombin is a glycoprotein with a molecular weight of 70 kDa and is composed of a single polypeptide chain. Prothrombin is activated by factors Va, Xa, and phospholipids to produce the serine protease thrombin[1]. | [Purification Methods]
Prothrombin is purified by two absorptions on a barium citrate adsorbent, followed by decomposition of the adsorbents with a weak carboxylic cation-exchanger (Amberlite IRF-97), isoelectric precipitation (pH 4.7-4.9) and further purification by chromatography on Sephadex G-200 or IRC-50. Finally it is recrystallised from a 1% solution adjusted to pH 6.0-7.0 and partial lyophilisation to ca 1/5 to 1/10th volume and set aside at 2-5o to crystallise. Occasionally seeding is required. [Miller Biochemical Preparations 13 49 1971.] | [References]
[1] Suttie JW, et al. Prothrombin structure, activation, and biosynthesis. Physiol Rev. 1977 Jan;57(1):1-70. DOI:10.1152/physrev.1977.57.1.1 |
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