| Identification | Back Directory | [Name]
ALPHA,ALPHA-TREHALOSE GLUCOHYDROLASE | [CAS]
9025-52-9 | [Synonyms]
TREHALASE
EC 3.2.1.28
α,α-trehalase
Native Porcine Trehalase
α,α-trehalose glucohydrolase
trehalase from porcine kidney
ALPHA,ALPHA-TREHALOSE GLUCOHYDROLASE
Trehalase from porcine kidney buffered aqueous glycerol solution, >=1.0 units/mg protein | [EINECS(EC#)]
805-523-5 | [MDL Number]
MFCD00132462 |
| Chemical Properties | Back Directory | [density ]
1.32-1.42g/cm3 at 20℃ | [storage temp. ]
-20°C | [form ]
buffered aqueous glycerol solution | [color ]
Colorless to light yellow | [Specific Activity]
≥1.0units/mg protein | [LogP]
-1.3 at 20℃ and pH5.7-5.8 |
| Hazard Information | Back Directory | [Uses]
Trehalase has been used:
- in a study to assess changes in carbohydrate metabolism in Plasmopara viticola-infected grapevine leaves
- in a study to investigate growth arrest by trehalose-6-phosphate
- to quantify the intracellular trehalose in Lactobacillus casei cells
| [General Description]
Trehalase is a glycosidase that occurs in many forms and is found in all tissues. It belongs to the glycoside hydrolase 37 (GH37) family of the carbohydrate-active enzyme (CAZy) classification. | [Biochem/physiol Actions]
Trehalase catalyzes the hydrolysis of trehalose to produce two glucose units. It mediates flight metabolism, molting induced chitin production and tolerance towards cold temperature. | [Purification Methods]
This trehalase is purified by solubilising in Triton X-100 and sodium deoxycholate, and submitting to gel filtration, ion-exchange chromatography, conA-Sepharose chromatography, phenyl-Sepharose CL-4B hydrophobic interaction chromatography, Tris-Sepharose 6B affinity and hydrolyapatite chromatography. Activity is increased 3000-fold. [Yoneyama Arch Biochem Biophys 255 168 1987.] T4-RNA ligase (from bacteriophage-infected E.coli ) Mr 43,500, [EC 6.5.1.3 for RNA lyase]. This ligase is purified by differential centrifugation and separation on a Sephadex A-25 column, then through hydroxylapatite and DEAE-glycerol using Aff-Gel Blue to remove DNAase activity. (Greater than 90% of the protein in the enzyme preparation migrated as a single band on gradient polyacrylamide gels containing SDS during electrophoresis.) [McCoy et al. Biochim Biophys Acta 562 149 1979.] |
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Sigma-Aldrich
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