| Identification | Back Directory | [Name]
3ALPHA-HYDROXYSTEROID DEHYDROGENASE | [CAS]
9028-56-2 | [Synonyms]
HSD
3αHSD
EC 1.1.1.50
EC: 1.1.1.50
3α-Hydroxysteroid
HYDROXYSTEROID OXIDOREDUCTASE
3α-Hydroxysteroid Dehydrogenase
Dehydrogenase, 3a-hydroxy steroid
3ALPHA-HYDROXYSTEROID DEHYDROGENASE
3-alpha-Hydroxysteroidedehydrogenase
3alpha-Hydroxysteroid oxidoreductase
Dehydrogenase, 3alpha-hydroxy steroid
3α-hydroxysteroid:nad(p)+ oxidoreductase
3ALPHA-HYDROXYSTEROID DEHYDROGENASE F. &
3α-Hydroxysteroid Dehydrogenase, 90 units/Mg
3α-Hydroxysteroid Dehydrogenase, Recombinant
3ALPHA-HYDROXYSTEROID: NAD[P]+ OXIDOREDUCTASE
3ALPHA-HYDROXYSTEROID DEHYDROGENASE USP/EP/BP
3α-hydroxysteroid 3-dehydrogenase (Re-specific)
3α-hydroxysteroid 3-dehydrogenase (Si-specific)
3α-Hydroxysteroid Dehydrogenase [>=50 units/mg]
3α-Hydroxysteroid Dehydrogenase, 28 kDa (SDS-PAGE)
3α-Hydroxysteroid Dehydrogenase froM MicroorganisM
3α-Hydroxysteroid Dehydrogenase From Microorganism
3A-HYDROXYSTEROID DEHYDROGENASE FROM*PSE UDOMONAS TE
3α-Hydroxysteroid Dehydrogenase, >=90 units/mg protein
3alpha-hydroxysteroid dehydrogenase F. pseu. test. ~9 U/mg
3α-hydroxysteroid dehydrogenase from pseudomonas testosteroni
Native Pseudomonas testosteroni 3α-Hydroxysteroid Dehydrogenase
3α-Hydroxysteroid Dehydrogenase from B. choshinensis, Recombinant
3alpha-Hydroxysteroid Dehydrogenase from Pseudomonas testosteroni | [EINECS(EC#)]
232-839-5 | [MDL Number]
MFCD00130298 |
| Chemical Properties | Back Directory | [storage temp. ]
2-8°C
| [form ]
ammonium sulfate suspension
| [color ]
White to off-white | [biological source]
bacterial (Pseudomonas testosteroni) | [Specific Activity]
≥15units/mg protein | [Cosmetics Ingredients Functions]
SKIN CONDITIONING |
| Hazard Information | Back Directory | [Description]
3α-Hydroxysteroid dehydrogenase is a key enzyme catalyzing steroid metabolism. Its core function is to reversibly catalyze the oxidation of 3α-hydroxysteroids to 3-oxosteroids, using NAD+ or NADP+ as cofactors. As a protein, its molecular weight and optimal reaction conditions vary depending on the source (e.g., human, rat, or bacterial) and subtype. For example, some human subtypes prefer to use NADPH as a cofactor and exhibit optimal activity in a near-neutral environment with a pH of 6.8–7.4. The purified product is typically a white powder. | [Chemical Properties]
Powder | [Uses]
3α-Hydroxysteroid Dehydrogenase from Pseudomonas testosterone has been used:
- in the preparation of composite electrode platform to for the determination of androsterone
- in potassium phosphate buffer for the bioluminescent assay of bile acids in serum
- for total bile salts assay using an enzymatic method
| [Application]
3α-Hydroxysteroid dehydrogenase (3α-HSD) plays a crucial physiological role in vivo, responsible for inactivating circulating steroid hormones and regulating their activity in target tissues (especially the liver), thus participating in steroid hormone clearance. In vitro, purified 3α-HSD is widely used as a biochemical reagent, primarily for clinical diagnosis and research. For example, it can be used to determine enzyme activity levels for quantitative analysis of bile acid or certain steroid hormone levels. Furthermore, it is an important tool for studying the mechanisms of action of nonsteroidal anti-inflammatory drugs (NSAIDs) and progestins, as these drugs are often found to be inhibitors of this enzyme. | [Biochem/physiol Actions]
3α-hydroxysteroid dehydrogenase (3α-HSD) may help to modulate the occupancy of the androgen receptor (AR) in androgen target tissues. It may also act in hindering androgen action. |
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