| Identification | Back Directory | [Name]
MYOGLOBIN | [CAS]
9047-17-0 | [Synonyms]
MYOGLOBIN
MYOGLOBIN, SPERM WHALE
MyoglobinExSpermWhaleSaltFree
MYOGLOBIN SPERM WHALE, RECOMBINANT
myoglobin from sperm whale skeletal muscle
Myoglobin (Physeter catodon protein moiety)
Myoglobin, sperm whale recombinant, from E. coli | [EINECS(EC#)]
232-935-7 | [MDL Number]
MFCD00164834 |
| Hazard Information | Back Directory | [Definition]
A globular protein occurringwidely in muscle tissue as anoxygen carrier. It comprises a singlepolypeptide chain and a haem group, which reversibly binds a moleculeof oxygen. This is only relinquishedat relatively low externaloxygen concentrations, e.g. duringstrenuous exercise when muscle oxygendemand outpaces supply fromthe blood. Myoglobin thus acts as anemergency oxygen store. | [Purification Methods]
Myoglobin is purified by CM-cellulose chromatography and Sephadex G-50 followed by chromatography on Amberlite IRC-50 Type III or BioRex 70 (<400mesh). The crystalline product as a paste in saturated (NH4)2SO4 at pH 6.5-7.0 may be stored at 4o for at least 4years unchanged, but must not be kept in a freezer. [Anres & Atassi Biochemistry 12 942 1980, Edmundson Biochemical Preparations 12 41 1968.] |
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