ChemicalBook--->CAS DataBase List--->916753-62-3

916753-62-3

916753-62-3 Structure

916753-62-3 Structure
IdentificationBack Directory
[Name]

Butanoic acid, 4-[[4-[2-[2,5-dimethoxy-4-[2-(4-nitrophenyl)diazenyl]phenyl]diazenyl]phenyl]methylamino]-, 2,5-dioxo-1-pyrrolidinyl ester
[CAS]

916753-62-3
[Synonyms]

BHQ-2 NHS
HCQ-2 NHS
HCQ-2 NHS (~BHQ-2 NHS)
BHQ-2-succinimide ester
2,5-Dioxopyrrolidin-1-yl 4-((4-((2,5-dimethoxy-4-((4-nitrophenyl)diazenyl)phenyl)diazenyl)phenyl)(methyl)amino)butanoate
Butanoic acid, 4-[[4-[2-[2,5-dimethoxy-4-[2-(4-nitrophenyl)diazenyl]phenyl]diazenyl]phenyl]methylamino]-, 2,5-dioxo-1-pyrrolidinyl ester
[Molecular Formula]

C29H29N7O8
[MOL File]

916753-62-3.mol
[Molecular Weight]

603.59
Chemical PropertiesBack Directory
[Boiling point ]

780.6±70.0 °C(Predicted)
[density ]

1.37±0.1 g/cm3(Predicted)
[solubility ]

易溶于DMSO, DMF, 甲醇等
[pka]

2.91±0.50(Predicted)
[Appearance]

Dark purple solid
[ex/em]

566 nm (absorbance peak)
[ε(extinction coefficient)]

38000 L⋅mol−1⋅cm−1
[InChIKey]

UTKREZFIUDGDHQ-RRPBDJRISA-N
[SMILES]

N(/C1C=C(C(=CC=1OC)/N=N/C1C=CC(=CC=1)N(=O)=O)OC)=N\C1C=CC(=CC=1)N(C)CCCC(=O)ON1C(CCC1=O)=O
Hazard InformationBack Directory
[Description]

UBQ-2 NHS Ester is a dark quencher with no native emission due to the polyaromatic-azo backbone and a terminal NHS ester. UBQ-2 NHS Ester has a wide and intense quenching range from 560-670 nm, which makes it useful as an acceptor in fluorescence resonance energy transfer (FRET) applications in conjunction with orange to far-red emitting dyes. The NHS ester can be applied to label the primary amines (-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules.
[Chemical Properties]

Appearance: Dark purple solid
ex/em: 566 nm (absorbance peak)
Extinction coefficient (ε): 38000 L??mol??1??cm??1
[Uses]

BHQ-2 NHS is a dark quencher with no native emission due to the polyaromatic-azo backbone and a terminal NHS ester. UBHQ-2 NHS has a wide and intense quenching range from 560-670 nm, which makes it useful as an acceptor in fluorescence resonance energy transfer (FRET) applications in conjunction with orange to far-red emitting dyes. The NHS ester can be applied to label the primary amines (-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules.
[Chemical Reactivity]

Primary amine
[Abs/Em Maxima]

580 nm
[Extinction Coefficient]

38,000 cm-1M-1
[Spectrally Similar Dyes]

Cy3, Cy5, ROX, Texas Red, Alexa Fluor® 546, 555, 594, 633, 647, IRDye® 650
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