| Identification | Back Directory | [Name]
Rp-8-bromo-Cyclic AMPS (sodium salt) | [CAS]
925456-59-3 | [Synonyms]
Rp-isomer
Rp-8-bromo-cAMPS
8-Bromoadenosine 3'
Rp-8-bromo-Cyclic AMPS
5'-cyclic monophosphorothioate
Rp-8-bromo-Cyclic AMPS (sodium salt)
RP-8-BR-CAMPS;8-BROMOADENOSINE 3';5'-CYCLIC MONOPHOSPHOROTHIOATE; RP-ISOMER;RP-8-BROMO-CAMPS;925456-59-3 | [Molecular Formula]
C10H12BrN5NaO5PS | [MOL File]
925456-59-3.mol | [Molecular Weight]
448.16 |
| Chemical Properties | Back Directory | [storage temp. ]
Store at -20°C | [solubility ]
DMF: 30 mg/ml; DMSO: 25 mg/ml; Ethanol: 0.5 mg/ml; PBS (pH 7.2): 10 mg/ml | [form ]
A crystalline solid |
| Hazard Information | Back Directory | [Description]
Rp-8-bromo-Cyclic AMPS (Rp-8-bromo-cAMPS) is a cell-permeable cAMP analog that combines an exocyclic sulfur substitution in the equatorial position of the cyclophosphate ring with a bromine substitution in the adenine base of cAMP.1,2 It acts as an antagonist of cAMP-dependent protein kinases (PKAs) and is resistant to hydrolysis by cyclic nucleotide phosphodiesterases. Rp-8-bromo-cAMPS more effectively antagonizes cAMP-dependent activation of purified PKA type I from rabbit muscle than PKA type II from bovine heart.2 | [Uses]
Rp-8-Br-cAMPS sodium is an analog of cAMP and an inhibitor of PKA. Rp-8-Br-cAMPS sodium occupies cAMP binding sites on PKA type I regulatory subunits, thereby preventing PKA dissociation and activation. Rp-8-Br-cAMPS sodium can be used in the study of tumors and retrovirus-induced immune deficiency. Rp-8-Br-cAMPS sodium also inhibits insulin secretion[1][2][3]. | [in vivo]
Rp-8-Br-cAMPS (1 mg; intraperitoneal injection; 10 days) improves immune function in a mouse retrovirus infection model[3]. | Animal Model: | Murine leukemia retrovirus RadLV-Rs treated male C57BL/6 mice[3] | | Dosage: | 1 mg | | Administration: | Intraperitoneal injection (i.p.); 10 days | | Result: | Had no significant effect on the extent of lymphadenopathy and splenomegaly which is typical of RadLV-Rs retroviral infection.
Strongly increased responses to the anti-CD3 mAb.
Improved T cell responses.
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| [storage]
Store at -20°C | [References]
1. Dostmann, W.R., Taylor, S.S., Genieser, H.G., et al. Probing the cyclic nucleotide binding sites of cAMP-dependent protein kinases I and II with analogs of adenosine 3',5'-cyclic phosphorothioates J. Biol. Chem. 265(18),10484-10491(1990).
2. Gjertsen, B.T., Mellgran, G., Otten, A., et al. Novel (Rp)-cAMPS analogs as tools for inhibition of cAMP-kinase in cell culture. Basal cAMP-kinase activity modulates interleukin-1β action J. Biol. Chem. 270(35),20599-20607(1995). |
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| Company Name: |
Biorbyt Ltd.
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| Tel: |
+44 (0)1223 859 353 |
| Website: |
http://www.biorbyt.com |
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