| Identification | Back Directory | [Name]
L-Lysinamide, N-[2-(7-methoxy-2-oxo-2H-1-benzopyran-4-yl)acetyl]-L-alanyl-L-valyl-L-leucyl-L-glutaminyl-L-serylglycyl-L-phenylalanyl-L-arginyl-N6-(2,4-dinitrophenyl)-L-lysyl- | [CAS]
932391-88-3 | [Synonyms]
L-Lysinamide, N-[2-(7-methoxy-2-oxo-2H-1-benzopyran-4-yl)acetyl]-L-alanyl-L-valyl-L-leucyl-L-glutaminyl-L-serylglycyl-L-phenylalanyl-L-arginyl-N6-(2,4-dinitrophenyl)-L-lysyl- | [Molecular Formula]
C69H99N19O20 | [MOL File]
932391-88-3.mol | [Molecular Weight]
1514.64 |
| Hazard Information | Back Directory | [Uses]
MCA-AVLQSGFR-Lys(Dnp)-Lys-NH2 is a fluorescent substrate used for fluorescence resonance energy transfer (FRET) protease assays. This substrate is employed to measure the activity of SARS-CoV-2 3CLpro[1]. | [Biological Activity]
SARS-CoV Mpro FRET substrate. The MCA-AVLQSGFR-Lys(Dnp)-Lys-NH2 substrate sequence is derived from residues P4–P5' of the SARS-CoV Mpro N-terminal autoprocessing site (AVLQSGFRK). Enzymatic cleavage between Q4 and S5 of MCA-AVLQSGFR-Lys(Dnp)-Lys-NH2 releases the highly fluorescent 7-methoxycoumarin (Mca) group from the influence of the 2,4-dinitrophenyl (Dnp) internal quencher resulting in a large fluorescence intensity increase. This peptide is suitable to measure protease activity of the Mpro & SARS-CoV Mpro COVID-19 viruses in addition to other viruses. | [storage]
-20°C | [References]
[1] Su HX, et al. Anti-SARS-CoV-2 activities in vitro of Shuanghuanglian preparations and bioactive ingredients. Acta Pharmacol Sin. 2020 Sep;41(9):1167-1177. DOI:10.1038/s41401-020-0483-6 |
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