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L-Homopropargylglycine (HPG) is a noncanonical amino acid analog of methionine that contains a terminal alkyne moiety. HPG-labeling is a fast, sensitive, non-toxic, and non-radioactive alternative to the traditional technique for detecting nascent protein synthesis.

HPG is the cell-permeable compound randomly incorporated into synthesizing protein instead of methionine during translation. The resulting alkyne-labeled full-length proteins can be detected via copper-catalyzed click reaction with fluorescent or biotin-labeled azides and used for subsequent microscopic imaging or purification tasks.