1160852-22-1

中文名称化合物 T12819

英文名称 S130

CAS 1160852-22-1

分子式 C24H25N3O2

分子量 387.47

MOL 文件 1160852-22-1.mol

1160852-22-1 结构式

基本信息物理化学性质化合物 T12819价格(试剂级) 常见问题列表

基本信息

中文别名

化合物 T12819

英文别名

S130
S130,S-130
7H-Dibenzo[de,g]quinoline-4-carboxamide, N-[3-(diethylamino)propyl]-7-oxo-

物理化学性质

沸点641.5±50.0 °C(Predicted)

密度1?+-.0.06 g/cm3(Predicted)

储存条件-20°C储存

溶解度DMSO : 125 mg/mL (322.61 mM; Need ultrasonic)

酸度系数(pKa)13.15±0.20(Predicted)

形态Solid

颜色White to off-white

化合物 T12819价格(试剂级)

报价日期	产品编号	产品名称	CAS号	包装	价格
2025/12/22	HY-112818	化合物 T12819 S130	1160852-22-1	1 mg	1590元
2025/12/22	HY-112818	暂无 S130	1160852-22-1	5mg	3500元
2025/12/22	HY-112818	暂无 S130	1160852-22-1	10mM * 1mLin DMSO	3850元

常见问题列表

生物活性

S130 是一种高亲和力、选择性的半胱氨酸蛋白酶 ATG4B 的抑制剂, IC50 值为 3.24 µM。S130 可以抑制自噬通量。

靶点

IC50: 3.24 µM (ATG4B)

体外研究

S130 suppresses autophagy and activates apoptosis by inhibiting ATG4B, leads to enhanced cytotoxicity.
S130 (10 μM; 6 hours) suppresses autophagy at the early LC3 priming step or late autolysosome degradation stage.
S130 accumulates autolysosomes with more lipidated LC3.
S130 (0-25 μM; 48 hours) induces cell death through inhibiting the activity of ATG4B at a dose higher than 6.3 µM. And such cytotoxicity might not cause cell death through necroptosis.
Nutrient deprivation enhances S130-induced cytotoxicity.
S130 (0-10μM; 24 hours) suppresses approximately 79% of the cleavage of full-length LC3-GST at the 10 µM, while no substrates were processed in ATG4B KO cells. S130 displays obvious inhibitory effects on ATG4B.

Cell Cytotoxicity Assay

Cell Line:	HeLa cells, HCT116 cells, HL60 cells
Concentration:	0 μM, 3.1 μM, 6.3 μM, 12.5 μM, 25 μM
Incubation Time:	48 hours
Result:	Had significant cytotoxic effects on HeLa cells (IC ₅₀ =16.1 µM), HCT116 cells(IC ₅₀ =9.0 µM) and HL60 cells (IC ₅₀ =4.7 µM) at a dose higher than 6.3 µM. And such cytotoxicity might not cause cell death through necroptosis.

Cell Autophagy Assay

Cell Line:	HeLa cells and MEF cells
Concentration:	10 μM
Incubation Time:	6 hours
Result:	Suppressed autophagy at the early LC3 priming step or late autolysosome degradation stage.

Western Blot Analysis

Cell Line:	HeLa cells
Concentration:	0 μM, 5 μM, 10 μM
Incubation Time:	24 hours
Result:	Suppressed approximately 79% of the cleavage of full-length LC3-GST at 10 µM, while no substrates were processed in ATG4B KO cells.

体内研究

S130 (20 mg/kg; i.p.; daily; 3 weeks) suppresses tumor growth, and shows an efficient in vivo antitumor effect with a sound safety on vital organs.

Animal Model:	BALB/c nude female mice (4 weeks), with HCT116 cells xenograft
Dosage:	20 mg/kg
Administration:	Intraperitoneal injection; daily; 3 weeks
Result:	Was able to suppress tumor growth and with a sound safety on vital organs.