157134-53-7
中文名称
DI-8-ANEPPS
英文名称
DI-8-ANEPPS
CAS
157134-53-7
分子式
C36H52N2O3S
分子量
592.87
MOL 文件
157134-53-7.mol
更新日期
2024/11/26 11:42:17
157134-53-7 结构式
基本信息
中文别名
膜电位荧光探针DI-8-ANEPPSDI-8-ANEPPS(膜电位荧光探针)
英文别名
Jpw 1153DI-8-ANEPPS
Di-8-ANEPPS *CAS#: 157134-53-7*
4-(2-[6-(DIOCTYLAMINO)-2-NAPHTHALENYL]ETHENYL)-1-(3-SULFOPROPYL)PYRIDINIUM INNER SALT
物理化学性质
外观性状外观 : 橘红色固体
ex/em : 468/633 nm (人工膜)
消光系数(ε) : 44,000 L⋅mol−1⋅cm−1 (甲醇)
ex/em : 468/633 nm (人工膜)
消光系数(ε) : 44,000 L⋅mol−1⋅cm−1 (甲醇)
熔点>200℃
储存条件2-8°C
溶解度可溶于DMSO
形态粉末
颜色橙色至红色
外观Orange solid
ε(消光系数)≥37 at 495-501 nm in methanol
激发峰(Ex)/发射峰(Em)468/633 nm (artificial membrane)
最大波长(λmax)498 nm
生物领域应用Measuring membrane potential
常见问题列表
荧光探针
Di-8-ANEPPS 是萘基苯乙烯基（ANEP）电压敏感染料，它们的荧光激发和发射光谱会因电压的变化而变化。相对于Di-4-ANEPPS，Di-8-ANEPPS有更长的碳链尾巴，更容易驻留在细胞膜双脂层的外层，可胜任长时间的电压检测实验。Di-8-ANEPPS比Di-4-ANEPPS光学性质更稳定，光毒性更小。用途
膜电位荧光探针生物活性
Di-8-ANEPPS 是萘基苯乙烯基电压敏感染料，它们的荧光激发和发射光谱会因电压的变化而变化。体外研究
A mouse cell staining with Di-8-ANEPPS is applied voltage clamp pulses and immersed in Na + -containing solution. The Di-8-ANEPPS signal, which largely reflects t system voltage, has an asymmetrical positive component upon application of depolarizing pulses. This is interpreted as reflecting a propagating action potential, escaping from the voltage-clamped plasma membrane. Its temporal properties are not unlike those of the “center” signal. Notably, the peak of the signal occurrs 0.3 ms after the leading edge of the depolarizing pulse.