210292-65-2

中文名称 NAPH-EA-MAL

英文名称 1H-Benz[de]isoquinoline-1,3(2H)-dione, 2-butyl-6-[[2-(2,5-dihydro-2,5-dioxo-1H-pyrrol-1-yl)ethyl]amino]-

CAS 210292-65-2

分子式 C22H21N3O4

分子量 391.42

MOL 文件 210292-65-2.mol

210292-65-2 结构式

基本信息物理化学性质 NAPH-EA-MAL价格(试剂级) 常见问题列表

基本信息

英文别名

Naph-EA-mal
Thiol-green 1
1H-Benz[de]isoquinoline-1,3(2H)-dione, 2-butyl-6-[[2-(2,5-dihydro-2,5-dioxo-1H-pyrrol-1-yl)ethyl]amino]-

物理化学性质

沸点633.5±45.0 °C(Predicted)

密度1.371±0.06 g/cm3(Predicted)

酸度系数(pKa)1.51±0.20(Predicted)

形态Solid

颜色Light yellow to orange

NAPH-EA-MAL价格(试剂级)

报价日期	产品编号	产品名称	CAS号	包装	价格
2025/12/22	HY-D1261	Naph-EA-mal	210292-65-2	1 mg	1363元
2025/12/22	HY-D1261	NAPH-EA-MAL Naph-EA-mal	210292-65-2	5 mg	3000元
2025/12/22	HY-D1261	Naph-EA-mal	210292-65-2	10 mM * 1 mLin DMSO	3300元

常见问题列表

生物活性

Naph-EA-mal (Thiol-green 1) 是一种快速检测和超快 turn-on 型硫醇 (thiol) 荧光探针，可用于蛋白标记和生物成像。Naph-EA-mal (Thiol-green 1) 可用于检测活细胞中的硫醇，标记蛋白质硫醇，定量细胞裂解液中总硫醇的浓度，并确定固定细胞中可逆的蛋白质硫醇氧化。Ex: 488 nM; Em: 540 nM。

体外研究

Imaging thiols in living cellsHep G2 cells incubated with Naph-EA-mal (Thiol-green 1) (1 µM;5 mins), and strong green fluorescence can be observed. When cells are preincubated the cells to block the intracellular thiols with NEM (a thiol block reagent; 100 µM), a weaker fluorescence is observed in cells.Monitoring thiol-disulfide levels in fixed cellsDue to the majority of cellular thiols are present in the reduced form, Naph-EA-mal (Thiol-green 1) (10 µM) induces strong fluorescence in Hep G2 cells. After diamide treatment, fluorescent signal sharp dimed is observed.The Hep G2 cells, oxidized by diamide (200 µM; 30 min), are fixed by 70% EtOH and the remaining free thiols are blocked by NEM (100 µM). then The cells are further treated with TCEP (5 mM) followed by addition of the Naph-EA-mal (Thiol-green 1) (10 µM). The green fluorescent signal is detected again