7240-37-1
7240-37-1 结构式
基本信息
7-氨基放线菌素D
7-氨基放线菌素D(7-AAD)
7-AAD [7-氨基放线菌素 D]
7-ADD
7-AMINO-AMD
7-AAD/CH85402
7-ACTINOMYCIN C
7-AMINOACTINOMYCIN
7-AMINODACTINOMYCIN
7-AMINOACTINOMYCIN D
7-AMINOACTINOMYCIN C1
ACTINOMYCIN D, 7-AMINO
物理化学性质
安全数据
常见问题列表
7-AAD选择性亲和DNA中GC区，可用于显影染色体中的条带区域。
7-AAD被报道具有抗菌活性，并对某些白血病细胞株有增殖抑制活性。
- 7-AAD为潜在致癌物，请避免与皮肤眼睛直接接触，操作中戴手套，穿实验服。
7-Aminoactinomycin D (7-AAD) is a DNA dye that distinguishes viable, apoptotic, and late apoptotic/dead cells in flow cytometry. 7-Aminoactinomycin D staining with 5 μg/mL, 10 μg/mL, and 20 μg/mL, but not with 1 μg/mL, is suitable for quantification of apoptosis in flow cytometry.
7-Aminoactinomycin D is frequently used to stain and exclude dead cells in flow cytometry at low concentrations (0.5-5 μg/mL). At higher concentrations (10-20 μg/mL), 7-Aminoactinomycin D has also been used to distinguish between viable cells (7-AAD
negative
) and apoptotic cells (7-AAD
dim
) or dead cells (7-AAD
bright
) using the fact that permeability of the cell membrane, and hence fluorescence intensity, is low in early apoptotic cells and high in late apoptotic and dead cells.