STING IFN-β
CCCP inhibits IFN-β production induced by various types of the STING pathway activators. CCCP suppresses the phosphorylation of STING, TBK1, and IRF3 via disrupting the association of STING and TBK1. CCCP inhibits activation of STING and its downstream signaling molecules, TBK1 and IRF3, but not STING translocation to the perinuclear region. CCCP impairs the interaction between STING and TBK1 and concomitantly triggers mitochondria fission. Importantly, the knockout of the crucial mitochondria fission regulator Drp1 restored the STING activity, indicating that CCCP down-modulates the STING pathway through DRP1-mediated mitochondria fragmentation. The protonophore CCCP that disrupts membrane potential suppresses the DMXAA-triggered STING signaling pathway. CCCP drastically suppresses the production of IFN-β in DMXAA-treated RAW264.7 cells and MEFs.
The same dosage of 3 mg/kg.bw each of CCCP and PPEF is used. In both the cases 1 log reduction is observed in the bacterial load. However, when 3 mg/kg.bw of PPEF is used in combination with 3 mg/kg.bw of CCCP, 6 log 10 reduction is observed in the bacterial count. The developed model validates the enhanced antibacterial activity of combination therapy. 99m Tc-MIBI signals in the hearts of SD rats administered CCCP (4 mg/kg intraperitoneally) or vehicle is also measured. 99m Tc-MIBI signals decrease in rat hearts administered CCCP, and the ATP content, as measured by 31 P magnetic resonance spectroscopy, decreased simultaneously. To investigate whether CCCP decreased the 99m Tc-MIBI signals in rats, we analyzed the radioisotope activity of excised heart tissue from rats administered CCCP. At 180 min after 99m Tc-MIBI injection, the 99m Tc-MIBI signals from the hearts in the CCCP group are significantly lower than those in the vehicle group.
108-42-9
109-77-3
555-60-2
向冰浴冷却的间氯苯胺（95.70 mmol，1当量）在水（5 mL/mmol）中的溶液中依次缓慢滴加37%盐酸水溶液（11当量）和1M亚硝酸钠水溶液（1当量）。将反应混合物在0℃下搅拌30分钟后，在持续搅拌下缓慢滴加到预先冷却至0℃的丙二腈（143.55 mmol，1.5当量）和醋酸钠（31当量）在水（8.5 mL/mmol间氯苯胺）中的溶液中。反应2小时后，过滤收集不溶性腙产物，并用冷水洗涤。将所得沉淀溶解于乙酸乙酯中，随后用饱和食盐水洗涤有机层。有机层经无水硫酸镁干燥后，减压浓缩除去溶剂。最后，通过硅胶柱色谱纯化，得到目标产物N-(3-氯苯基)羰基亚肼基二氰化物。
参考文献：
[1] Synthesis (Germany), 2016, vol. 48, # 24, p. 4569 - 4579