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Evans Blue

Evans Blue structure

CAS No.: 314-13-6

Chemical Name:: Evans Blue

Synonyms: t1824;evablin;CI 23860;C.I.24075;DiazoBlue;blue Evan;Evans Blu;diazobleu;Evens Blue;EVANS BLUE

CBNumber:: CB1161546

Molecular Formula:: C34H29N6NaO14S4

Molecular Weight:: 896.86

MDL Number:: MFCD00004021

MOL File:: 314-13-6.mol

MSDS File:: SDS

TDS File:: TDS

Last updated:2026-05-15 07:54:27

Product description	Number	Pack Size	Price
Evans Blue Dye content ≥75%	E2129	10g	$73.4
Evans Blue Dye content ≥75%	E2129	50g	$292
Evans Blue >80.0%(HPLC)	E0197	25g	$55
Evans Blue	44513	100mg	$25
Evans Blue	44513	500mg	$119
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Evans Blue Properties

storage temp.	Store at RT.
solubility	DMSO (Slightly), Water (Slightly)
Colour Index	23860
form	Powder/Solid
color	Dark Brown
Water Solubility	280 g/L (20 ºC)
Merck	14,3905
Stability	Stable. Incompatible with strong reducing agents, strong oxidizing agents.
Major Application	diagnostic assay manufacturing hematology histology
InChIKey	AFPPOOOTZAGPEB-DVDDBBOFSA-N
SMILES	NC1=C(C=C(S(O)(=O)=O)C2=CC=C(/N=N/C3C=CC(C4C=CC(/N=N/C5C=CC6=C(C=C(S(O)(=O)=O)C(N)=C6C=5O)S(O)(=O)=O)=C(C)C=4)=CC=3C)C(O)=C12)S(O)(=O)=O.[NaH]
CAS DataBase Reference	314-13-6
FDA UNII	45PG892GO1
IARC	3 (Vol. 8, Sup 7) 1987
EPA Substance Registry System	C.I. Direct Blue 53 (314-13-6)
UNSPSC Code	12171500
NACRES	NA.47

SAFETY

Risk and Safety Statements

Symbol(GHS)

GHS08

Signal word

Danger

Hazard statements

H350

Precautionary statements

P201-P202-P280-P308+P313-P405-P501

Hazard Codes

T,F

Risk Statements

45-63-11-61-20/21/22

Safety Statements

53-45-36/37-16-36/37/39

WGK Germany

RTECS

QJ6440000

TSCA

TSCA listed

HS Code

32129000

Storage Class

6.1C - Combustible acute toxic Cat.3
toxic compounds or compounds which causing chronic effects

Hazard Classifications

Carc. 1B

Toxicity

LD50 intraperitoneal in mouse: 340mg/kg

NFPA 704

	1
1		0

Evans Blue price More Price(32)

Manufacturer	Product number	Product description	CAS number	Packaging	Price	Updated	Buy
Sigma-Aldrich	E2129	Evans Blue Dye content ≥75%	314-13-6	10g	$73.4	2026-04-30	Buy
Sigma-Aldrich	E2129	Evans Blue Dye content ≥75%	314-13-6	50g	$292	2026-04-30	Buy
TCI Chemical	E0197	Evans Blue >80.0%(HPLC)	314-13-6	25g	$55	2026-04-30	Buy
Cayman Chemical	44513	Evans Blue	314-13-6	100mg	$25	2026-04-30	Buy
Cayman Chemical	44513	Evans Blue	314-13-6	500mg	$119	2026-04-30	Buy

Product number	Packaging	Price	Buy
E2129	10g	$73.4	Buy
E2129	50g	$292	Buy
E0197	25g	$55	Buy
44513	100mg	$25	Buy
44513	500mg	$119	Buy

Evans Blue Chemical Properties,Uses,Production

Chemical Properties

Evans Blue is a blue crystal with a green metallic luster. It is very soluble in water, slightly soluble in ethanol, and almost insoluble in benzene, chloroform, and ether. It is soluble in acids and alkalis. It is hygroscopic, inducing moisture. It changes color when the pH is close to 10.

Uses

Evans Blue is used for estimation of blood volume and in double-labeling procedure for studying axonal branching. It acts as an inhibitor of L-glutamate and kainate receptor-mediated currents. It is also a P2X-selective purinoceptor antagonist. It is also useful in molecular biology.

Definition

ChEBI: Evans blue is an organic sodium salt that is the tetrasodium salt of 6,6'-{(3,3'-dimethyl[1,1'-biphenyl]-4,4'-diyl)bis[diazene-2,1-diyl]}bis(4-amino-5-hydroxynaphthalene-1,3-disulfonate). It is sometimes used as a counterstain, especially in fluorescent methods to suppress background autofluorescence. It has a role as a histological dye, a fluorochrome, a teratogenic agent and a sodium channel blocker. It contains an Evans blue(4-).

Preparation

3,3′-Dimethylbenzidine double nitriding, in alkaline conditions and 4-Amino-5-hydroxynaphthalene-1,3-disulfonic acid (2 Moore) coupled.

General Description

Blue crystals with a greenish-bronze luster or a black powder.

Air & Water Reactions

Azo dyes can be explosive when suspended in air at specific concentrations. Soluble in water.

Reactivity Profile

Direct Blue 53 is an azo compound. Azo, diazo, azido compounds can detonate. This applies in particular to organic azides that have been sensitized by the addition of metal salts or strong acids. Toxic gases are formed by mixing materials of this class with acids, aldehydes, amides, carbamates, cyanides, inorganic fluorides, halogenated organics, isocyanates, ketones, metals, nitrides, peroxides, phenols, epoxides, acyl halides, and strong oxidizing or reducing agents. Flammable gases are formed by mixing materials in this group with alkali metals. Explosive combination can occur with strong oxidizing agents, metal salts, peroxides, and sulfides. Direct Blue 53 can react with strong reducing and oxidizing agents.

Fire Hazard

Flash point data for Direct Blue 53 are unavailable; however, Direct Blue 53 is probably combustible.

Biological Activity

Potent inhibitor of L-glutamate uptake into synaptic vesicles. Also inhibits AMPA and kainate receptor-mediated currents (IC 50 values are 220 and 150 nM respectively). P2X-selective purinoceptor antagonist.

Safety Profile

Poison by intraperitoneal route.Moderately toxic by intravenous route. An experimentalteratogen. Other experimental reproductive effects.Questionable carcinogen with experimental tumorigenicdata. Mutation data reported. When heated todecomposition it

in vitro

evans blue inhibits the kainate-mediated responses of the non-nmda receptor subunits (giurl, g1ur1,2, g1url,3, and g1ur2,3) expressed in xenopus oocytes without the response of glur3 and g1ur6 at low concentrations (ic50= 355 nm for the subunit combination glur1,2). this pocess was partially reversible without competing with kainate for the agonist binding site [1]. in whole-cell patch clamp recordings of transfected human embryonic kidney 293 cells, evans blue is a potent inhibitor of glutamate-evoked currents mediated by the kainate-type receptor giur6 as long as the ampa-type receptor glurl. interestingly, pretreating with the lectin concanavalin cells recorded relatively little eb inhibition of giur6 currents, eliminating kainate receptor desensitization. in addition to decreasing glur6-mediated peak current amplitude, eb significantly changed receptor desensitization by slowing the rate of onset by ~2-fold (1 m eb) and the rate of recovery by ~2-fold (0.1 p.m eb), and enhancing the steady state to peak current amplitude ratio by ~50-fold (1 m eb) [2].

IC 50

220 and 150 nm for glurl and giur6, respectively.

storage

Room temperature

Staining Procedures

Evans Blue (3,7-Diamino-5-phenylphenazinium chloride) is used as a vital stain. It is used in a 0.05-0.25% aqueous solution for 5 minutes. Living cells remain unstained, while dead cells become blue.

Properties and Applications

dark green light blue. Soluble in water for bright blue. The strong sulfuric acid for blue-ray green, diluted for light yellow. The dye solution to join hydrochloric acid as bright blue; Add sodium hydroxide solution for the purple and red.

Standard	Acid Resistance	Alkali Resistance	Light Fastness	Soaping		Water
				Fading	Stain	Fading	Stain
ISO	5	4-5	1-2	2		2

References

[1] roseth s, fykse em, fonnum f. uptake of l-glutamate into rat brain synaptic vesicles: effect of inhibitors that bind specifically to the glutamate transporter. j neurochem. 1995 jul;65(1):96-103.
[2] price cj, raymond la. evans blue antagonizes both alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate and kainate receptors and modulates receptor desensitization. mol pharmacol. 1996 dec; 50 (6):1665-71.

Evans Blue Preparation Products And Raw materials

Raw materials

O-Tolidine 4-amino-5-hydroxynaphthalene-1,3-disulphonic acid

Preparation Products

Evans Blue Suppliers

Global( 411)Suppliers

Supplier	Tel	Email	Country	ProdList	Advantage
Henan Tianfu Chemical Co.,Ltd.	+86-0371-55170693 +86-19937530512	info@tianfuchem.com	China	21588	55
career henan chemical co	+86-0371-86658258 +8613203830695	sales@coreychem.com	China	29816	58
CONIER CHEM AND PHARMA LIMITED	+8618523575427	sales@conier.com	China	49977	58
career henan chemical co	+86-0371-86658258 +8613203830695	factory@coreychem.com	China	29794	58
SIMAGCHEM CORP	+86-5922680277 +86-13806087780	sale@simagchem.com	China	17346	58
TargetMol Chemicals Inc.	+1-781-999-5354; +17819995354	marketing@targetmol.com	United States	32467	58
Hefei TNJ Chemical Industry Co.,Ltd.	+86-0551-65418671 +8618949823763	sales@tnjchem.com	China	34563	58
ANHUI WITOP BIOTECH CO., LTD	+8615255079626	eric@witopchemical.com	China	23541	58
Shaanxi Dideu Medichem Co. Ltd	+86-029-89586680 +86-18192503167	1026@dideu.com	China	10000	58
Dayang Chem (Hangzhou) Co.,Ltd.	+86-0571-88938639 +8617705817739	info@dycnchem.com	China	52705	58

Supplier	Advantage
Henan Tianfu Chemical Co.,Ltd.	55
career henan chemical co	58
CONIER CHEM AND PHARMA LIMITED	58
career henan chemical co	58
SIMAGCHEM CORP	58
TargetMol Chemicals Inc.	58
Hefei TNJ Chemical Industry Co.,Ltd.	58
ANHUI WITOP BIOTECH CO., LTD	58
Shaanxi Dideu Medichem Co. Ltd	58
Dayang Chem (Hangzhou) Co.,Ltd.	58

View Lastest Price from Evans Blue manufacturers

Update time	Product	Price	Purity	Supply Ability	Manufacturer
2026-05-16	Evans blue 314-13-6	US $29.00 / mL	86.41%	10g	TargetMol Chemicals Inc.
2026-05-15	Evans blue 314-13-6	US $29.00 / mL	86.41%	10g	TargetMol Chemicals Inc.
2026-05-15	Evans blue 314-13-6	US $29.00 / mL	86.41%	10g	TargetMol Chemicals Inc.

Evans blue
314-13-6
US $29.00 / mL
86.41%
TargetMol Chemicals Inc.

Evans blue
314-13-6
US $29.00 / mL
86.41%
TargetMol Chemicals Inc.

Evans blue
314-13-6
US $29.00 / mL
86.41%
TargetMol Chemicals Inc.

Evans Blue Spectrum

Direct Blue 53(314-13-6)¹HNMR Direct Blue 53(314-13-6)IR1 Direct Blue 53(314-13-6)IR2

314-13-6(Evans Blue)Related Search:

Dichlorodiphenylsilane 2,2'-Azodi(2-methylbutyronitrile) Diphenylsilane Chlorodiphenylphosphine Azodicarbonamide

Diphenylphosphine Dacthal Dimethyl ether Azobenzene ETHANE

Dimethyl carbonate Dimethyl fumarate Biphenyl Diisopropyl azodicarboxylate tert-Butylchlorodiphenylsilane

Dimethyl sulfoxide N,N-Dimethylformamide 4-AMINOAZOBENZENE

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EVANS BLUE C.I. No 23860 EvansBlue,99% DiazoBlue 1,3-Naphthalenedisulfonic acid, 6,6-(3,3-dimethyl1,1-biphenyl-4,4-diyl)bis(azo)bis4-amino-5-hydroxy-, tetrasodium salt 4,4'-Bis[7-(1-amino-8-hydroxy-2,4-disulfo)naphthylazo]-3,3'-bitolyl tetrasodium salt 6,6'-[(3,3'-Dimethyl-4,4'-biphenylylene)bis(azo)]bis[4-amino-5-hydroxy-1,3-naphthalenedisulfonic acid] tetrasodium salt 6,6'-[(3,3'-Dimethyl[1,1'-biphenyl]-4,4'-diyl)bis(azo)]bis(4-amino-5-hydroxy-1,3-naphthalenedisulfonic acid disodium) 3-[[4'-[(4-Amino-2-methyl-5-methoxyphenyl)azo]-3,3'-dimethoxy-1,1'-biphenyl-4-yl]azo]-4-hydroxynaphthalene-1,5-disulfonic acid disodium salt C.I.24075 6,6-([(3,3'-DIMETHYL)-1,1'-BIPHENYL]-4,4'-DIYL)BIS(AZO)BIS[4-AMINO-5-HYDROXY-1,3-NAPHTHALENEDISULPHONIC ACID] TETRASODIUM SALT EVANS BLUE TETR Evans Blue,Direct Blue 53 EVANS BLUE COUNTERSTAIN EVANS BLUE, FOR MICROSCOPY blue Evan evans blue (C.I. 23860) evans blue fluka Evens Blue tetrasodium 6,6-((3,3-dimethyl-(1,1-biphenyl-4,4diyl)bis(azo)bis(4-amino-5-hydroxy-1,3-naphthalenedisulphonate) tetrasodium 6,6'-((3,3'-dimethyl-(1,1'-biphenyl-4,4'diyl)bis(azo)bis(4-amino-5-hydroxy-1,3-naphthalenedisulphonate) Evans Blue 5GR EVANS BLUE EVAN'S BLUE EVANS BLUE PURE EVANS BLUE TETRASODIUM SALT GEIGY BLUE DIRECT BLUE 53 CI NO 23860 CI 23860 1,3-naphthalenedisulfonicacid,6,6’-((3,3’-dimethyl-4,4’-biphenylylene)bis(azo 1,3-naphthalenedisulfonicacid,6,6’-[(3,3’-dimethyl[1,1’-biphenyl]-4,4’-diyl)b 1,3-Naphthalenedisulfonicacid,6,6’-[[3,3’-dimethyl(1,1’-biphenyl)-4,4’-diyl]bis(azo)]bis(4-amino-5-hydroxy-,tetrasodiumsalt 4,4’-bis(1-amino-8-hydroxy-2,4-disulfo-7-naphthylazo)-3,3’-bitolyl,tetrasodi 4,4’-bis(1-amino-8-hydroxy-2,4-disulpho-7-naphthylazo)-3,3’-bitolyl,tetrasod 4,4’-bis(7-(1-amino-8-hydroxy-2,4-disulfo)naphthylazo)-3,3’-bitolyl,tetrasod 4,4’-bis(7-(1-amino-8-hydroxy-2,4-disulpho)naphthylazo)-3,3’-bitolyl,tetraso 4,4’-bis-[7-(1-Amino-8-hydroxy-2,4-disulpho-naphthyl)azo]-3,3-bitolylsodiumsalt Evans Blu 6,6’-[3,3’-Dimethyl(1,1’-biphenyl)-4,4’-diyl]bis(azo)-bis-[4-amino-5-hydroxy-1,3-naphthale-nedisulfo] azovanblue blekitevansa C.I.DirectBlue5,3-tetrasodiumsalt c.i.directblue53 c.i.directblue53,tetrasodiumsalt diamineskyblueff diazobleu diazolpurebluebf directblue53(evan’s directblue53(evan’sblue) dyeevansblue evablin Evans blue, Dye content: &bsim:85% 6,6-([(3,3'-DIMETHYL)-1,1'-BIPHENYL]-4,4'-DIYL)BIS(AZO)BIS[4-AMINO-5-HYDROXY-1,3-NAPHTHALENEDISULPHONIC ACID] TETRASODIUM SALT 3,3'-DITOLYL-4,4'-BIS[7-AZO-1-AMINO-8-NAPHTHOL-2,4-(DISODIUM)DISULFONATE] evansblue,sodiumsalt evansbluedye geigy-blau536 geigyblue536,med